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Identifying, Diagnosing, and Grading Malignant Peripheral Nerve Sheath Tumors in Genetically Engineered Mouse Models
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Validation of tissue microarray technology in malignant peripheral nerve sheath tumours.

K Soares Gonçalves Cunha1, A Cunha Caruso, A Soares Gonçalves

  • 1Postgraduate Program of Pathology, School of Medicine, Fluminense Federal University (UFF), Rio de Janeiro, Brazil. karingoncalves@terra.com.br

Journal of Clinical Pathology
|March 26, 2009
PubMed
Summary

Tissue microarrays (TMAs) are reliable for studying malignant peripheral nerve sheath tumours (MPNSTs). Immunohistochemical analysis of MPNSTs using TMAs showed good agreement with whole tissue sections, validating TMA use.

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Area of Science:

  • Oncology
  • Pathology
  • Biotechnology

Background:

  • Tissue microarrays (TMAs) are increasingly used in research.
  • Concerns exist regarding the representativeness of donor tissue cores in TMAs.
  • This study addresses the validity of TMAs for studying specific tumour types.

Purpose of the Study:

  • To validate the use of tissue microarray (TMA) technology for immunohistochemical (IHC) studies of malignant peripheral nerve sheath tumours (MPNSTs).
  • To assess the concordance between TMA core results and whole tissue section results for MPNSTs.

Main Methods:

  • A TMA was created with five cores from 14 formalin-fixed, paraffin-embedded MPNSTs.
  • Immunohistochemistry (IHC) was performed using anti-Ki-67 and anti-S-100 antibodies.
  • Digital image analysis quantified positive staining; kappa statistics assessed agreement between TMA cores and whole sections.

Main Results:

  • Good to very good agreement was found between TMA cores and whole sections for MPNSTs.
  • Using at least four cores, 92% agreement (kappa = 0.77) was achieved for S-100 protein.
  • For Ki-67, 86% agreement (kappa = 0.63) was observed with at least four readable cores.

Conclusions:

  • Tissue microarray (TMA) technology is validated for immunohistochemical (IHC) analysis of malignant peripheral nerve sheath tumours (MPNSTs).
  • TMAs are suitable for MPNST studies, even when using heterogeneous markers like S-100 protein.