Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Anthelminthic Agents01:15

Anthelminthic Agents

Anthelmintic drugs differ significantly from antiparasitic therapies targeting protozoa, primarily due to differences in parasite biology. Whereas most protozoal treatments act on proliferating cells, anthelmintics are typically directed against mature, nonproliferative helminths. The therapeutic approach considers the helminth's reliance on neuromuscular coordination, glucose metabolism, and microtubular integrity for survival, reproduction, and localization within the host. Most anthelmintics...
Cardiovascular Drugs: Classification based on Therapeutic Indications01:18

Cardiovascular Drugs: Classification based on Therapeutic Indications

Cardiovascular diseases, encompassing a range of conditions, can significantly affect the heart's operations and the overall circulatory system. These conditions impair the heart's ability to pump blood, leading to a deficit in oxygen supply to crucial organs. Anomalies in the heart's electrical system, known as arrhythmias, can cause heartbeats to accelerate or slow down. Usually, heart rates increase during physical activity and decrease while resting or sleeping. However, frequent irregular...
Physical Properties of Amines01:26

Physical Properties of Amines

Amines with low molecular weight are usually gaseous at room temperature, while those with high molecular weight are liquid or solids in nature. Usually, low molecular weight amines have a rotten fish-like smell. Diamines typically have a pungent smell. For instance, cadaverine and putrescine, depicted in Figure 1, are two molecules responsible for decaying tissue.
Chemotherapy-Induced Nausea and Vomiting: Neurokinin-1 Receptor Antagonists01:28

Chemotherapy-Induced Nausea and Vomiting: Neurokinin-1 Receptor Antagonists

Neurokinin 1 (NK1) receptors are distributed across the GI tract, vagal afferents, and key CNS regions including the central vomiting center and chemoreceptor trigger zone (CTZ) Chemotherapy agents stimulate enterochromaffin cells in the gastrointestinal (GI) tract to release large amounts of substance P (SP). SP is a neuropeptide released by specific sensory nerves in response to many different stressors, including those in the GI mucosa affected by chemotherapy.  SP binds and activates these...
Drugs that Destabilize Microtubules01:10

Drugs that Destabilize Microtubules

Microtubules are dynamic structures and can be regulated by microtubule targeting agents (MTAs). Microtubule destabilizing drugs are a class of MTAs that destabilize and prevent microtubules' polymerization. Both natural and synthetic chemicals can be found under this class of drugs. Vincristine and vinblastine, two vinca alkaloids, and colchicine were among the first to be discovered. These drugs can affect cells in various ways, either by inducing a change in cell morphology, preventing...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Targeting USP8 with odoroside A regulates LXRβ-mediated fatty acid metabolic reprogramming against colorectal cancer.

Acta pharmacologica Sinica·2026
Same author

Virulensome: a transient organelle mediating fungal virulence.

Science China. Life sciences·2026
Same author

First-Line Tislelizumab Combined with Bevacizumab and CAPOX for Metastatic Gastroesophageal Adenocarcinoma with Low Programmed Death-Ligand 1 Expression.

Cancer communications (London, England)·2026
Same author

<i>DhSip1</i>, an NRPS-independent siderophore biosynthetic gene, regulates adhesive knob formation and pathogenicity in <i>Dactylellina haptotyla</i> through mediating iron acquisition.

Applied and environmental microbiology·2026
Same author

A study on the prevalence and influencing factors of the willingness of healthy subjects to participate in clinical trials.

Frontiers in public health·2026
Same author

Blood group antigens and malaria susceptibility.

Frontiers in cellular and infection microbiology·2025

Related Experiment Video

Updated: Jun 24, 2026

Culturing and Screening the Plant Parasitic Nematode Ditylenchus dipsaci
08:04

Culturing and Screening the Plant Parasitic Nematode Ditylenchus dipsaci

Published on: January 31, 2022

Nematicidal cardenolides from Nerium indicum Mill.

Xing-Biao Wang1, Guo-Hong Li, Li-Jun Zheng

  • 1Laboratory for Conservation and Utilization of Bio-Resource, and Key Laboratory for Microbial Resources of the Ministry of Education, Yunnan University, Kunming, P. R. China.

Chemistry & Biodiversity
|March 26, 2009
PubMed
Summary

Three cardenolides from Nerium indicum, including a new compound, show nematicidal activity against plant and free-living nematodes. This research highlights cardenolides as potential natural nematicides.

Related Experiment Videos

Last Updated: Jun 24, 2026

Culturing and Screening the Plant Parasitic Nematode Ditylenchus dipsaci
08:04

Culturing and Screening the Plant Parasitic Nematode Ditylenchus dipsaci

Published on: January 31, 2022

Area of Science:

  • Natural Product Chemistry
  • Nematology
  • Pharmacognosy

Background:

  • Cardenolides are a class of naturally occurring compounds with diverse biological activities.
  • Plant-derived compounds are increasingly explored for their potential as natural pesticides.
  • Nematodes cause significant agricultural damage and pose challenges for control.

Purpose of the Study:

  • To isolate and identify nematicidal compounds from Nerium indicum.
  • To evaluate the efficacy of isolated cardenolides against various nematode species.
  • To report the nematicidal activity of cardenolides for the first time.

Main Methods:

  • Bioassay-guided fractionation of the AcOEt extract of Nerium indicum.
  • Isolation and structural elucidation of three cardenolides, including one novel compound.
  • Determination of median lethal concentrations (LC50) against Bursaphelenchus xylophilus, Panagrellus redivivus, and Caenorhabditis elegans.

Main Results:

  • A new cardenolide (1) and two known compounds (uzarigenin (2) and cardenolide N-1 (3)) were isolated.
  • Compounds 1-3 exhibited varying degrees of nematicidal activity against the tested nematode species.
  • LC50 values ranged from 29.1 to 257.0 mg/l, indicating significant activity for some compounds.

Conclusions:

  • Nerium indicum contains cardenolides with potent nematicidal properties.
  • The novel compound 3beta-O-(beta-D-diginosyl)-14,15alpha-dihydroxy-5alpha-card-20(22)-enolide shows promising nematicidal activity.
  • Cardenolides represent a potential new class of natural nematicides for agricultural applications.