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Related Concept Videos

Preparation of Samples for Electron Microscopy01:20

Preparation of Samples for Electron Microscopy

To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does not destroy them and dried thoroughly (desiccated/dehydrated) so the vacuum does not affect them. Fixation needs to be done as quickly as possible because the sample properties will start changing as soon as it is removed from its natural environment. For example, in a tissue sample, the oxygen levels begin decreasing, causing an altered...

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Micromechanical Tension Testing of Additively Manufactured 17-4 PH Stainless Steel Specimens
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Published on: April 7, 2021

Double microtensile bond strength test: an alternative methodology for sample preparation.

Luca Giachetti1, Claudia Bambi, Michele Nieri

  • 1Department of Dentistry, Faculty of Medicine and Surgery, University of Florence, Viale Morgagni 85, Florence, Italy. l.giachetti@odonto.unifi.it

European Journal of Oral Sciences
|March 27, 2009
PubMed
Summary

This study compared a standard microtensile bond strength (muTBS) test with a new method. The alternative approach demonstrated higher bond strength and easier handling for dental composite testing.

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Area of Science:

  • Dental Materials Science
  • Adhesive Dentistry
  • Biomaterials Testing

Background:

  • The microtensile bond strength (muTBS) test is crucial for evaluating dental adhesive performance.
  • Traditional muTBS methods can present challenges in specimen preparation and handling.
  • Optimizing muTBS protocols is essential for accurate assessment of dentin bonding.

Purpose of the Study:

  • To compare the conventional microtensile bond strength (muTBS) test with a modified technique.
  • To evaluate the feasibility and advantages of an alternative method for assessing dentin bond strength.

Main Methods:

  • Six human molars were sectioned to create two specimen types: dentin-slab (DS) and sectioned-tooth (SS).
  • A self-etch adhesive and resin composite were applied, and specimens were sectioned into multiple sticks.
  • Both DS and SS specimens were subjected to tensile testing using a multilevel statistical model.

Main Results:

  • Specimens prepared using the modified SS method exhibited a significantly higher maximum load at break compared to DS specimens.
  • The modified methodology facilitated easier manipulation of test sticks.
  • The modified method allowed for effective testing of deep dentin samples.

Conclusions:

  • The modified muTBS methodology offers practical advantages, including simplified specimen handling.
  • This alternative approach provides a reliable means for evaluating the bond strength of dental adhesives to deep dentin.
  • The enhanced SS specimen preparation method shows promise for improving the efficiency and accuracy of muTBS testing.