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Updated: Jun 24, 2026
Longitudinal Micro-Computed Tomography Image Analysis for User-Defined Region of Interest in Critical-Sized Bone Defects
Published on: June 24, 2025
Micro-CT analysis of alveolar bone healing using a rat experimental model of critical-size defects
H Ebina1, J Hatakeyama, M Onodera
1Division of Periodontology and Endodontology, Tohoku University Graduate School of Dentistry, Sendai, Japan.
Objective:
This study was designed to establish a rat model of a critical size alveolar bone defect.
Materials And Methods:
Standardized buccal or mesiobuccal alveolar bone defects were made around the right first mandibular molar of 12-week-old rats, and the left was used as a control. Alveolar bone healing was examined quantitatively by three-dimensional micro-computed tomographic imaging. Bone matrix production of osteoblasts and osteocytes during repair of alveolar bone defects was examined with in situ hybridization for type I collagen.
Results:
Buccal defects were repaired significantly and the volume decreased by 88.3% in week 24, whereas mesiobuccal defects were repaired little. Osteoblasts and osteocytes expressed type I collagen in both defects in week 3 but showed little expression by week 6 and thereafter, leaving the mesiobuccal defects largely unrepaired.
Conclusion:
The mesiobuccal defect is a critical-size defect that is not ultimately repaired with bone. It may be an appropriate experimental model for investigating the effectiveness of bone regenerative agents in human alveolar bone loss.
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