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Updated: Jun 24, 2026

Radiochemical Assessment of Glycogen Synthase Enzyme Activity in Animal Tissue
Published on: October 24, 2025
Characterization of the interaction between latency-associated nuclear antigen and glycogen synthase kinase 3beta
1Department of Biochemistry, National University of Singapore, Singapore. bchth@nus.edu.sg
Abstract:
The latency-associated nuclear antigen (LANA) of Karposi's sarcoma-associated herpesvirus has been reported to interact with glycogen synthase kinase 3beta (GSK-3beta) and regulate its activity, leading to inhibition of GSK-3-dependent beta-catenin degradation. In this study, the interaction between LANA and GSK-3beta was characterized further. LANA was found to interact with GSK-3beta in vitro as well as in intact cells. However, LANA did not regulate GSK-3beta kinase activity and LANA-induced upregulation of beta-catenin was GSK-3beta independent. LANA did not regulate the stability of beta-catenin or of its reported interaction partners p53 and von Hippel-Lindau protein. Additional targets of LANA are likely to mediate its malignancy-promoting function.
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