Prominin-1/CD133 marks stem cells and early progenitors in mouse small intestine
View abstract on PubMed
Summary
This summary is machine-generated.Prominin-1 (Prom1) marks intestinal stem cells and transit-amplifying progenitors. This indicates Prom1 is not a specific marker solely for Lgr5(+) intestinal stem cells.
Area Of Science
- Gastrointestinal biology
- Stem cell research
- Cellular lineage tracing
Background
- Prominin-1 (Prom1)/CD133 is a known marker for stem cells in various adult tissues.
- Leucine-rich-repeat-containing G-protein-coupled receptor 5 (Lgr5) marks intestinal stem cells responsible for gastrointestinal epithelium regeneration.
- The relationship between Prom1 and Lgr5 in the intestine requires investigation.
Purpose Of The Study
- To investigate the intestinal expression pattern of Prominin-1 (Prom1)/CD133.
- To determine if Prom1 is a specific marker for Lgr5(+) intestinal stem cells.
- To visualize and trace Prom1-expressing cells in the intestine.
Main Methods
- Analysis of Prom1 mRNA and protein expression in intestinal crypt stem cells.
- Generation of Prominin-1 (Prom1)/CD133 knock-in mice (Prom1(-mCherry-IRES-CreERT2) KI) for cell tracing.
- Utilizing tamoxifen-inducible CreERT2 recombinase for genetic lineage tracing of Prom1-positive cells.
Main Results
- Prom1 mRNA expression was observed throughout the lower half of intestinal crypts.
- Prom1 protein was detected on Lgr5(+) intestinal stem cells and transit-amplifying progenitors.
- Prominin-1 (Prom1) marks a broader stem cell/transit-amplifying progenitor compartment than previously recognized.
Conclusions
- Prominin-1 (Prom1) is expressed in both intestinal stem cells and transit-amplifying progenitors.
- Prominin-1 (Prom1) is not a specific marker exclusively for Lgr5(+) intestinal stem cells.
- The findings refine the understanding of cell surface markers in intestinal stem cell identification.