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Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers

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Published on: April 9, 2014

Infrared multiphoton microscopy: subcellular-resolved deep tissue imaging.

Volker Andresen¹, Stephanie Alexander, Wolfgang-Moritz Heupel

¹University of Würzburg, Rudolf-Virchow Center for Experimental Biomedicine, Department of Dermatology, Venerology, and Allergology, Würzburg, Germany.

Current Opinion in Biotechnology

|March 28, 2009

Summary

This summary is machine-generated.

Infrared multiphoton microscopy (IR-MPM) enhances deep tissue imaging by enabling red fluorophores, doubling imaging depth, and reducing phototoxicity. This advanced technique offers subcellular resolution for long-term live cell and deep tissue studies.

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Area of Science:

Biomedical optics
Microscopy techniques
Cellular imaging

Background:

Multiphoton microscopy (MPM) is crucial for deep tissue and live cell imaging.
Conventional MPM faces limitations in imaging depth and phototoxicity.

Purpose of the Study:

To present the setup and applications of infrared multiphoton microscopy (IR-MPM).
To highlight the advantages of IR-MPM over conventional MPM for biological imaging.

Main Methods:

Utilized excitation wavelengths above 1080 nm for IR-MPM.
Investigated the use of red fluorophores and fluorescent proteins.
Evaluated imaging depth, second harmonic generation, phototoxicity, and photobleaching.

Main Results:

IR-MPM enables the use of red fluorophores and fluorescent proteins.
Doubled imaging depth compared to conventional MPM.
Improved second harmonic generation of tissue structures.
Significantly reduced phototoxicity and photobleaching.
Achieved subcellular resolution at depths of several hundred micrometers.

Conclusions:

IR-MPM offers significant advantages for deep tissue and live cell microscopy.
The technique enhances imaging capabilities by increasing depth and reducing photodamage.
IR-MPM is a valuable tool for long-term live cell and deep tissue investigations.