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Preparation of Thermoresponsive Nanostructured Surfaces for Tissue Engineering

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Published on: March 1, 2016

Temperature-responsive intelligent interfaces for biomolecular separation and cell sheet engineering.

Kenichi Nagase¹, Jun Kobayashi, Teruo Okano

¹Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, TWIns, 8-1 Kawada-cho, Shinjuku, Tokyo 162-8666, Japan.

Journal of the Royal Society, Interface

|March 28, 2009

Summary

Intelligent surfaces made with temperature-responsive polymers offer tunable interactions for biomolecules and cells. These advanced materials enable novel applications in chromatographic separation and tissue engineering via cell sheet recovery.

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Area of Science:

Materials Science
Biomedical Engineering
Polymer Chemistry

Background:

Intelligent surfaces modified with poly(N-isopropylacrylamide) exhibit temperature-dependent hydrophilic/hydrophobic changes.
These alterations modulate interactions with biomolecules and cells, enabling smart material applications.
This review focuses on the use of these surfaces in chromatography and cell culture.

Purpose of the Study:

To review the applications of temperature-responsive intelligent surfaces in chromatographic separation and cell culture.
To discuss the key factors influencing separation efficiency and cell adhesion/detachment.
To summarize new methodologies for cell sheet culturing and tissue construction.

Main Methods:

Modification of interfaces with poly(N-isopropylacrylamide) and its derivatives.
Investigation of temperature-responsive hydrophilic/hydrophobic alterations.
Analysis of factors like wettability, copolymer composition, and graft polymer architecture for chromatography.
Evaluation of temperature-dependent cell adhesion/detachment for tissue engineering.

Main Results:

Intelligent surfaces facilitate thermally modulated interactions with biomolecules and cells.
Wettability, copolymer composition, and graft polymer architecture significantly impact chromatographic separation of bioactive compounds.
Temperature-responsive substrates enable the recovery of confluent cell monolayers as contiguous living cell sheets.
Effective control over cell adhesion/detachment is achieved through grafting temperature-responsive polymers.

Conclusions:

Temperature-responsive intelligent surfaces are versatile tools for biomedical applications, particularly in chromatography and tissue engineering.
These surfaces offer precise control over biomolecular interactions and cell behavior through external temperature stimuli.
Advancements in polymer grafting and cell culturing techniques pave the way for sophisticated tissue engineering strategies.