Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...
Induced Pluripotent Stem Cells01:06

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic cells are...
Anaphase Promoting Complex00:50

Anaphase Promoting Complex

The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein.
Master Transcription Regulators02:23

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
Epigenetic Regulation01:37

Epigenetic Regulation

Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Quercetin Affects Carcinogenic Phenotype of Breast and Lung Cancer Cells Differentially Through Sterol Regulation Mediated by MALAT1 Perturbation.

Chemistry, an Asian journal·2026
Same author

ESIPT-Active Naphthalene-Based Hydroxyoxime Fluorescent Chemodosimeters for Rapid and Sensitive Phosgene Sensing in Solution and Polymer Films.

Chemistry, an Asian journal·2026
Same author

Blue Vitriol Catalyzed Decarboxylative C-C Cross-Coupling of α,β-Unsaturated Acids for C-H Functionalization of Unactivated Ethers.

Organic letters·2026
Same author

Organelle communication networks rewire to support lipid metabolism during neuronal differentiation.

bioRxiv : the preprint server for biology·2026
Same author

Gene Therapy for Sickle Cell Anemia in India - Current Status and Challenges.

Indian journal of hematology & blood transfusion : an official journal of Indian Society of Hematology and Blood Transfusion·2026
Same author

Rewriting Genetic Destiny: Prime Editing Leads the Future in Fixing Genetic Disorders.

The National medical journal of India·2026

Related Experiment Video

Updated: Jun 24, 2026

CD Spectroscopy to Study DNA-Protein Interactions
06:48

CD Spectroscopy to Study DNA-Protein Interactions

Published on: February 10, 2022

Elevated polyamines induce c-MYC overexpression by perturbing quadruplex-WC duplex equilibrium.

Niti Kumar1, Richa Basundra, Souvik Maiti

  • 1Proteomics and Structural Biology Unit, Institute of Genomics and Integrative Biology, CSIR, Mall Road, Delhi 110 007, India.

Nucleic Acids Research
|March 28, 2009
PubMed
Summary

Polyamines interact with c-MYC quadruplex DNA, stabilizing its structure. This interaction promotes c-MYC gene expression, offering potential new cancer treatment strategies.

More Related Videos

Toxicological Assays for Testing Effects of an Epigenetic Drug on Development, Fecundity and Survivorship of Malaria Mosquitoes
10:26

Toxicological Assays for Testing Effects of an Epigenetic Drug on Development, Fecundity and Survivorship of Malaria Mosquitoes

Published on: January 16, 2015

Focus Formation: A Cell-based Assay to Determine the Oncogenic Potential of a Gene
08:18

Focus Formation: A Cell-based Assay to Determine the Oncogenic Potential of a Gene

Published on: December 31, 2014

Related Experiment Videos

Last Updated: Jun 24, 2026

CD Spectroscopy to Study DNA-Protein Interactions
06:48

CD Spectroscopy to Study DNA-Protein Interactions

Published on: February 10, 2022

Toxicological Assays for Testing Effects of an Epigenetic Drug on Development, Fecundity and Survivorship of Malaria Mosquitoes
10:26

Toxicological Assays for Testing Effects of an Epigenetic Drug on Development, Fecundity and Survivorship of Malaria Mosquitoes

Published on: January 16, 2015

Focus Formation: A Cell-based Assay to Determine the Oncogenic Potential of a Gene
08:18

Focus Formation: A Cell-based Assay to Determine the Oncogenic Potential of a Gene

Published on: December 31, 2014

Area of Science:

  • Molecular Biology
  • Biophysics
  • Genetics

Background:

  • Quadruplexes and polyamines are linked to cancer.
  • The interaction between quadruplexes and polyamines in transcriptional regulation is not well understood.

Purpose of the Study:

  • To investigate the role of polyamines in quadruplex structure and function.
  • To understand how polyamine-mediated changes in quadruplex structure affect gene expression, specifically c-MYC.

Main Methods:

  • Biophysical techniques including Circular Dichroism (CD), UV spectroscopy, and Förster Resonance Energy Transfer (FRET).
  • Analysis of polyamine effects on c-MYC quadruplex conformation, stability, and transition to duplex DNA.
  • Investigation of polyamine-induced structural changes on c-MYC gene expression.

Main Results:

  • Polyamines alter the conformation and stability of the c-MYC quadruplex.
  • Polyamines delay the transition from quadruplex to duplex DNA, favoring the quadruplex structure.
  • Polyamines induce a structural transition in the c-MYC quadruplex, leading to increased c-MYC gene expression.

Conclusions:

  • Polyamines play a critical role in regulating c-MYC expression through structural modulation of quadruplex DNA.
  • The findings suggest that targeting quadruplex-polyamine interactions could be a novel antiproliferative strategy for cancer therapy.