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Pristane induced arthritis. II. Genetic regulation in F1 hybrid mice and cellular immune abnormalities following
J M Chapdelaine1, J D Whalen, P H Wooley
1Department of Internal Medicine, Wayne State University Medical School, Detroit, MI 48201.
Abstract:
Pristane-induced arthritis was investigated in DBA/1, DBA/2, and BALB/c mice, and F1 hybrid mice generated from inter-crosses between these strains. The incidence of disease in F1 hybrid mice was significantly lower than the susceptible parental strains (DBA/1 and BALB/c), and resistance to arthritis was observed in both DBA/2 mice and the (DBA/2 x BALB/c) F1 hybrid mice. Several cellular immune abnormalities were observed in pristane-injected DBA/1 mice. Con A mitogen responses were depressed following pristane injection, and a functional suppressor cell population was detected. Delayed type hypersensitivity responses to type II collagen were observed in pristane injected mice. The intraperitoneal injection of pristane appears to alter immune regulation and induce autoimmune responses to connective tissue components.
Insights
Pristane induced arthritis in mice, revealing genetic resistance in DBA/2 strains and F1 hybrids. Immune abnormalities, including suppressed responses and delayed hypersensitivity, were noted in susceptible DBA/1 mice, suggesting altered immune regulation.
Area of Science:
- Immunology
- Autoimmunity
- Genetics
Background:
- Pristane is a hydrocarbon known to induce autoimmune diseases in susceptible mouse strains.
- Understanding the genetic basis and immune mechanisms of pristane-induced arthritis is crucial for autoimmune disease research.
Purpose of the Study:
- To investigate the genetic susceptibility and immune responses to pristane-induced arthritis in different mouse strains.
- To identify cellular immune abnormalities associated with pristane injection.
Main Methods:
- Pristane was administered intraperitoneally to DBA/1, DBA/2, and BALB/c mice, as well as their F1 hybrids.
- Disease incidence and severity were assessed.
- Cellular immune responses, including Concanavalin A (Con A) mitogen responses and delayed type hypersensitivity (DTH) to type II collagen, were evaluated.
Main Results:
- DBA/2 mice and (DBA/2 x BALB/c) F1 hybrid mice exhibited resistance to pristane-induced arthritis.
- F1 hybrid mice showed significantly lower disease incidence compared to susceptible parental strains (DBA/1 and BALB/c).
- Pristane-injected DBA/1 mice displayed depressed Con A mitogen responses, a functional suppressor cell population, and DTH responses to type II collagen.
Conclusions:
- Genetic background significantly influences susceptibility to pristane-induced arthritis.
- Pristane administration alters immune regulation, leading to autoimmune responses against connective tissue components.
- Cellular immune abnormalities observed in susceptible mice provide insights into the pathogenesis of this autoimmune condition.