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Synthesis of Cyclic Polymers and Characterization of Their Diffusive Motion in the Melt State at the Single Molecule Level

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Published on: September 26, 2016

Polymer translocation in a double-force arrangement.

S T T Ollila¹, K F Luo, T Ala-Nissila

¹Department of Applied Physics, Helsinki University of Technology, P.O. Box 1100, FIN-02015 TKK, Espoo, Finland. santtu.ollila@tkk.fi

The European Physical Journal. E, Soft Matter

|March 28, 2009

Summary

This study explores polymer translocation through nanopores using a double-force method for biopolymer sequencing. While slower, this method offers better control over polymer movement during translocation.

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Area of Science:

Biophysics
Polymer Physics
Nanotechnology

Background:

Polymer translocation through nanopores is crucial for biopolymer sequencing.
External forces are used to drive and control this process.
A double-force arrangement has been proposed for enhanced dynamical control.

Purpose of the Study:

To investigate the translocation dynamics of a driven polymer chain through a nanopore under a double-force arrangement.
To analyze the effect of opposing forces on translocation time and control.
To provide data for estimating sequencing errors in double-force experiments.

Main Methods:

Langevin dynamics simulations were employed.
A pulling force was applied to the first monomer.
An opposing force was applied within the pore.

Main Results:

Translocation is slower in the double-force arrangement compared to a single pulling force.
The scaling of translocation time with chain length (tau ~ N^2) remains unchanged.
Waiting times for monomer exit increase with bead number, indicating controlled velocity.

Conclusions:

The double-force arrangement allows for better dynamical control of polymer translocation.
This method shows promise for precise biopolymer sequencing.
Understanding translocation dynamics is key to minimizing sequencing errors.