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Updated: Jun 24, 2026

Rapid Detection of Fecal Antigen of Helicobacter pylori Infection Based on Double Antibody Sandwich Detection Technology
Published on: May 23, 2025
Increased cerebrospinal fluid Helicobacter pylori antibody in Alzheimer's disease
Jannis Kountouras1, Marina Boziki, Emmanuel Gavalas
1Department of Medicine, Second Medical Clinic, Aristotle University of Thessaloniki, Ippokration Hospital, Thessaloniki, Greece. jannis@med.auth.gr
Background:
Helicobacter pylori (H. pylori) infection may play a role in Alzheimer's disease (AD).
Aim:
A prospective, nonrandomized, comparative study was car- ried out to examine the levels of anti-H. pylori-specific IgG antibodies in the cerebrospinal fluid (CSF) and serum of AD patients, compared with those of age-matched cognitively normal controls.
Patients:
CSF was aspirated from 27 AD patients and 27 age-matched cognitively normal patients with prostate hyperplasia or long-bone fractures necessitating surgery after epidural anesthesia. Serum samples were obtained from AD patients and the day before surgery from controls.
Methods:
CSF and serum anti-H. pylori IgG concentrations were measured by means of an enzyme-linked immunosorbent assay.
Results:
The mean concentration of anti-H. pylori-specific IgG was significantly greater in (a) the CSF of AD patients (10.53 +/- 12.54 U/mL) than in controls (8.63 +/- 8.01 U/mL, p = 0.047), and (b) the serum of AD patients (30.44 +/- 33.94 U/mL) than in controls (16.24 +/- 5.77 U/mL, p = 0.041). CSF anti-H. pylori IgG antibodies correlated with the degree of severity of the disease.
Conclusion:
H. pylori-specific IgG antibody levels are significantly increased in CSF and serum of AD; its titer in CSF might reflect the AD severity, thereby supporting a role for this common infection in the pathobiology of the disease.
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