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Methods for Evaluating the Role of c-Fos and Dusp1 in Oncogene Dependence
Published on: January 7, 2019
Dihydrofolate reductase gene expression characterized by the PCR assay in human leukemia cells
H Miyachi1, H Han, K J Scanlon
1Department of Medical Oncology, City of Hope National Medical Center, Duarte, CA 91010.
In Vivo (Athens, Greece)
|January 1, 1991
Abstract:
A modified enzymatic amplification (PCR) method has been used to detect overexpression of the dihydrofolate reductase gene in human leukemic cells resistant to methotrexate (MTX) from both patient samples and cell lines in culture. Quantitation of DHFR mRNA by the PCR assay has been demonstrated to be more accurate and more sensitive than Northern blotting. This assay be useful in screening patients relapsing with drug resistance.

