Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Western Blotting01:15

Western Blotting

Western blotting is an analytical technique for protein identification. It has various applications in immunology and medicine, including detecting diseases like bovine spongiform encephalopathy, mad cow disease, and human and feline immunodeficiency virus from biological samples.
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Population-Level Risks for HIV Mortality During the COVID-19 Pandemic in the United States by Demographic Characteristics and Medicaid Access, 2020‒2021.

American journal of public health·2025
Same author

SARS-CoV-2 Infection Among Pregnant People at Labor and Delivery and Changes in Infection Rates in the General Population: Lessons Learned From Illinois.

Public health reports (Washington, D.C. : 1974)·2022
Same author

Acquired HIV drug resistance and virologic monitoring in a HIV hyper-endemic setting in KwaZulu-Natal Province, South Africa.

AIDS research and therapy·2021
Same author

Geographic and demographic heterogeneity of SARS-CoV-2 diagnostic testing in Illinois, USA, March to December 2020.

BMC public health·2021
Same author

Geographic and demographic heterogeneity of SARS-CoV-2 diagnostic testing in Illinois, USA, March to December 2020.

medRxiv : the preprint server for health sciences·2021
Same author

Community Transmission of SARS-CoV-2 Associated with a Local Bar Opening Event - Illinois, February 2021.

MMWR. Morbidity and mortality weekly report·2021

Related Experiment Video

Updated: Jun 24, 2026

Whole Blood Assay with Dual Co-Stimulation for Antigen-Specific Analysis of Host Immunity to Fungal and Viral Pathogens
06:03

Whole Blood Assay with Dual Co-Stimulation for Antigen-Specific Analysis of Host Immunity to Fungal and Viral Pathogens

Published on: September 20, 2024

Indeterminate HIV western blot test result.

Deborah L Barnett, Wayne A Duffus

    Journal of the South Carolina Medical Association (1975)
    |March 31, 2009
    PubMed
    Summary

    Explaining uncertain test results, like indeterminate HIV tests, can be uncomfortable for providers. Regular practice improves provider comfort, facilitating earlier patient care and better health outcomes.

    Area of Science:

    • Medical diagnostics
    • Public health

    Background:

    • Indeterminate Western Blot (IWB) results create diagnostic uncertainty.
    • Effective communication strategies are crucial for healthcare providers when discussing sensitive results.
    • Early linkage to care significantly improves health outcomes for patients with HIV.

    Observation:

    • Provider discomfort in explaining IWBs can be overcome with practice.
    • HIV is a manageable condition with early intervention.
    • Timely patient care is essential for preventing further HIV transmission.

    Findings:

    • Increased provider practice correlates with greater comfort in discussing uncertain test results.
    • Early diagnosis and care initiation lead to improved patient prognosis.
    • Prompt connection to treatment mitigates disease progression and transmission.

    More Related Videos

    Amplifying and Quantifying HIV-1 RNA in HIV Infected Individuals with Viral Loads Below the Limit of Detection by Standard Clinical Assays
    13:58

    Amplifying and Quantifying HIV-1 RNA in HIV Infected Individuals with Viral Loads Below the Limit of Detection by Standard Clinical Assays

    Published on: September 26, 2011

    Conformational Evaluation of HIV-1 Trimeric Envelope Glycoproteins Using a Cell-based ELISA Assay
    07:10

    Conformational Evaluation of HIV-1 Trimeric Envelope Glycoproteins Using a Cell-based ELISA Assay

    Published on: September 14, 2014

    Related Experiment Videos

    Last Updated: Jun 24, 2026

    Whole Blood Assay with Dual Co-Stimulation for Antigen-Specific Analysis of Host Immunity to Fungal and Viral Pathogens
    06:03

    Whole Blood Assay with Dual Co-Stimulation for Antigen-Specific Analysis of Host Immunity to Fungal and Viral Pathogens

    Published on: September 20, 2024

    Amplifying and Quantifying HIV-1 RNA in HIV Infected Individuals with Viral Loads Below the Limit of Detection by Standard Clinical Assays
    13:58

    Amplifying and Quantifying HIV-1 RNA in HIV Infected Individuals with Viral Loads Below the Limit of Detection by Standard Clinical Assays

    Published on: September 26, 2011

    Conformational Evaluation of HIV-1 Trimeric Envelope Glycoproteins Using a Cell-based ELISA Assay
    07:10

    Conformational Evaluation of HIV-1 Trimeric Envelope Glycoproteins Using a Cell-based ELISA Assay

    Published on: September 14, 2014

    Implications:

    • Healthcare providers should be trained in communicating uncertain diagnostic results.
    • Promoting widespread HIV screening is vital for early detection.
    • Integrating patients into care swiftly enhances individual health and community well-being.