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[Considerations regarding the nosocomial potential of surgical wards]
Monica Licker1, A Anghel, Anca Tutelcă
1UMF Victor Babe Timişoara, Disciplina de Microbiologie.
Purposes:
The aim of our study was to determine the prevalence of multiresistant germs with nosocomial potential and their main resistance phenotype and genotype patterns in surgical departments.
Methods:
Identification of germs was performed by the API system (BioMerieux France) and susceptibility tests by disk-diffusion tests, (CLSI standards) with automatic reading methods (Osiris-Bio Rad Laboratories). ESBL producing E. coli and Klebsiella pneumoniae strains have been also genotyped.
Results:
From 190 samples (urines, wound secretions, blood, etc.); we isolated 106 microbial strains with nosocomial potential. 56 (52.83%) from these strains were represented by enterobacteria, 26 (24.52%) by Gram negative non-fermentative rods, and 24 (22.64%) by Gram positive cocci.
Conclusions:
We noticed a high prevalence of multidrug resistant germs (ESBL, MRSA, etc). The majority of them were involved in nosocomial surgical site and urinary tract infections.
Insights
This study found a high prevalence of multidrug-resistant germs in surgical departments, often causing nosocomial infections. Understanding resistance patterns is key to combating these dangerous hospital-acquired infections.
Area of Science:
- Medical Microbiology
- Infectious Diseases
- Hospital Epidemiology
Background:
- Nosocomial infections pose a significant threat in surgical settings.
- Multidrug-resistant (MDR) germs are a growing concern in healthcare environments.
- Understanding resistance mechanisms is crucial for effective treatment and prevention.
Purpose of the Study:
- To determine the prevalence of MDR germs with nosocomial potential in surgical departments.
- To identify the primary resistance phenotype and genotype patterns of these germs.
- To assess the role of MDR germs in surgical site and urinary tract infections.
Main Methods:
- Germ identification using the API system (BioMerieux).
- Antimicrobial susceptibility testing via disk-diffusion (CLSI standards) with automatic reading (Osiris-Bio Rad).
- Genotyping of ESBL-producing E. coli and Klebsiella pneumoniae strains.
Main Results:
- 106 microbial strains with nosocomial potential were isolated from 190 samples.
- Enterobacteria comprised 52.83% of isolates, followed by Gram-negative non-fermentative rods (24.52%) and Gram-positive cocci (22.64%).
- A high prevalence of MDR germs, including ESBL and MRSA, was observed.
Conclusions:
- MDR germs are highly prevalent in surgical departments.
- These MDR germs are frequently implicated in nosocomial surgical site and urinary tract infections.
- The findings highlight the need for robust infection control measures and surveillance of resistance patterns.
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