Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA Isolation01:24

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Integrated hydrogel system targeting AMPK/PPARĪ³ signaling in osteoarthritis immunometabolic regulation.

Journal of nanobiotechnologyĀ·2026
Same author

NanoLoop: A Deep Learning Framework Leveraging Nanopore Sequencing for Chromatin Loop Prediction.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)Ā·2026
Same author

Network pharmacology-based elucidation of the regulatory mechanism of Solanum lyratum (Bai Ying) against psoriasis via the IL-17A/STAT3 axis: Molecular docking and HaCaT cell validation.

Pakistan journal of pharmaceutical sciencesĀ·2026
Same author

Cu-Co Dual Single Atom and Nitrogen Doped Carbon Nanotubes as Oxygen Reduction Reaction Electrocatalysts.

ACS applied materials & interfacesĀ·2026
Same author

An atypical presentation of NSCLC in a nonsmoker: Shoulder pain and upper extremity DVT: A case report.

MedicineĀ·2025
Same author

Breaking the energy density barrier in polymer film capacitors via molecular and interfacial design.

Journal of colloid and interface scienceĀ·2025

Related Experiment Video

Updated: Jun 24, 2026

A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons
11:40

A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons

Published on: November 14, 2018

Microfluidic DNA amplification--a review.

Yonghao Zhang1, Pinar Ozdemir

  • 1Department of Mechanical Engineering, University of Strathclyde, Glasgow, G1 1XJ, UK. yonghao.zhang@strath.ac.uk

Analytica Chimica Acta
|March 31, 2009
PubMed
Summary
This summary is machine-generated.

Microfluidic devices offer advanced DNA amplification, particularly continuous-flow polymerase chain reaction (PCR) on-chip. Microdroplet technology further enhances PCR by preventing contamination and enabling single DNA molecule amplification.

More Related Videos

Simple Bulk Readout of Digital Nucleic Acid Quantification Assays
06:55

Simple Bulk Readout of Digital Nucleic Acid Quantification Assays

Published on: September 24, 2015

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis
14:53

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

Published on: September 10, 2014

Related Experiment Videos

Last Updated: Jun 24, 2026

A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons
11:40

A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons

Published on: November 14, 2018

Simple Bulk Readout of Digital Nucleic Acid Quantification Assays
06:55

Simple Bulk Readout of Digital Nucleic Acid Quantification Assays

Published on: September 24, 2015

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis
14:53

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

Published on: September 10, 2014

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Analytical Chemistry

Background:

  • Microfluidic devices are increasingly studied for DNA amplification.
  • Polymerase chain reaction (PCR) is a fundamental technique in molecular biology.

Purpose of the Study:

  • To review the development of microfluidic PCR devices.
  • To discuss the physical principles for optimal microfluidic PCR design and operation.
  • To highlight microdroplet technology for on-chip PCR.

Main Methods:

  • Review of existing literature on microfluidic PCR devices.
  • Discussion of physical principles governing microfluidic device design.
  • Focus on continuous-flow and microdroplet-based PCR on-chip.

Main Results:

  • Continuous-flow microfluidic PCR can be integrated into micro-total-analysis systems.
  • Microdroplet technology addresses challenges like sample carryover and surface adsorption.
  • Microdroplet PCR eliminates chimeric products and shortens thermal cycling times.

Conclusions:

  • Microfluidic PCR, especially in droplets, shows great potential for single DNA molecule and single-cell amplification.
  • Chip-based droplet PCR offers significant advantages over traditional methods.
  • Further development of microfluidic PCR is crucial for advancing molecular diagnostics.