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Updated: Jun 24, 2026

Analysis of Somatic Hypermutation in the JH4 intron of Germinal Center B cells from Mouse Peyer's Patches
09:35

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Published on: April 20, 2021

Somatic hypermutation in peritoneal B1b cells.

Bishnudeo Roy1, Swati Shukla, Marcin Łyszkiewicz

  • 1Molecular Immunology, HZI, Helmholtz Centre for Infection Research, Inhoffenstr. 7, D-38124 Braunschweig, Germany. Bishnudeo.Roy@helmholtz-hzi.de

Molecular Immunology
|March 31, 2009
PubMed
Summary
This summary is machine-generated.

Peritoneal B1b cells, but not B1a or B2 cells, produce IgA and undergo somatic hypermutation. This suggests B1b cells bridge innate and adaptive immunity, similar to B2 cells.

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Last Updated: Jun 24, 2026

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09:35

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Assessing Somatic Hypermutation in Ramos B Cells after Overexpression or Knockdown of Specific Genes
08:12

Assessing Somatic Hypermutation in Ramos B Cells after Overexpression or Knockdown of Specific Genes

Published on: November 1, 2011

Area of Science:

  • Immunology
  • Cell Biology

Background:

  • Murine B1 cells are known to switch to IgA in vitro.
  • The presence and function of IgA-producing B1 cells in vivo remain less understood.

Purpose of the Study:

  • To investigate the presence and characteristics of IgA-producing B1 cells in the mouse peritoneum.
  • To compare IgA production and somatic hypermutation in different peritoneal B cell subpopulations (B1a, B1b) and splenic B2 cells.

Main Methods:

  • Enzyme-linked immunospot (ELISPOT) assays on lipopolysaccharide-stimulated peritoneal cells.
  • RT-PCR on sorted peritoneal B cell subpopulations.
  • Analysis of variable region mutations in IgA and IgM.

Main Results:

  • High numbers of IgA-producing B1b cells were detected in the mouse peritoneum.
  • IgA-producing B cells were rare in B1a and B2 cell populations.
  • Peritoneal B1b cells showed extensive somatic hypermutation in IgA variable regions, similar to B2 cells, while B1a cells showed low mutation frequencies.

Conclusions:

  • Peritoneal B1b cells are a distinct subpopulation capable of IgA class switching and somatic hypermutation.
  • These findings support a model of a layered immune system with B1b cells acting as intermediates between B1a and B2 cells.