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Updated: Jun 24, 2026

Integration of Bioinformatics Approaches and Experimental Validations to Understand the Role of Notch Signaling in Ovarian Cancer
Published on: January 12, 2020
Relationship between vascular endothelial growth factor and Notch1 expression and lymphatic metastasis in tongue
Young-Hoon Joo1, Chan-Kwon Jung, Min-Sik Kim
1Department of Otolaryngology, Head and Neck Surgery, College of Medicine, The Catholic University of Korea, Seoul, Korea.
Objective:
To determine the role of angiogenesis in lymph node metastasis and the depth of invasion in early tongue cancer.
Study Design:
Retrospective analysis.
Subjects And Methods:
The study included 51 subjects with tongue cancer. Immunohistochemical staining for vascular endothelial growth factor, Notch1, and Notch3 was performed. Microvessel density was evaluated by counting the number of CD34-stained microvessels in each pathologic specimen.
Results:
Significant correlations were found between vascular endothelial growth factor and Notch1 expression and cervical lymph node metastasis (P = 0.020 and P < 0.009, respectively), tumor depth of invasion (P = 0.001 and P < 0.001, respectively), and microvessel density indicated by CD34 staining (P = 0.001 and P < 0.001, respectively). Nodal metastasis (P = 0.022), T stage (P = 0.002), and positive VEGF expression (P = 0.044) were statistically significant prognostic factors for disease-specific survival.
Conclusion:
Vascular endothelial growth factor and Notch1 expression are significantly related to cervical lymph node metastasis and depth of invasion in tongue cancer patients.
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