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Related Concept Videos

Protein Glycosylation01:25

Protein Glycosylation

Glycosylation, the most common post-translational modification for proteins, serves diverse functions. Adding sugars to proteins makes the proteins more resistant to proteolytic digestion. Glycosylated proteins can act as markers and receptors to promote cell-cell adhesion. Additionally, they have many essential quality control functions in the cell, such as correct protein folding and facilitating transport of misfolded proteins to the cytosol, which can be degraded.
Glycosylation occurs in...
Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...

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Glycomics-Guided Glycoproteomics Facilitates Comprehensive Profiling of the Glycoproteome in Complex Tumor Microenvironments
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Glycoproteomics: past, present and future.

Bérangère Tissot1, Simon J North, Alessio Ceroni

  • 1Division of Molecular Biosciences, Faculty of Natural Sciences, Imperial College London, London SW7 2AZ, United Kingdom.

FEBS Letters
|March 31, 2009
PubMed
Summary
This summary is machine-generated.

This review covers glycoproteomics, the study of protein glycosylation, from its 1970s origins to current mass spectrometry techniques. It highlights analytical methods and informatics tools like GlycoWorkBench for complex glycoproteomic data analysis.

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Area of Science:

  • Proteomics
  • Mass Spectrometry
  • Glycobiology

Background:

  • Protein glycosylation, or glycoproteomics, emerged in the 1970s with mass spectrometry's application to protein sequencing.
  • Glycoproteomics involves analyzing the complex carbohydrate structures attached to proteins.

Purpose of the Study:

  • To review the historical development of glycoproteomics.
  • To describe current analytical approaches and informatics tools in glycoproteomics.
  • To present case studies illustrating glycoproteomic analysis.

Main Methods:

  • Review of historical literature and current research in glycoproteomics.
  • Description of analytical techniques for glycoproteomic analysis.
  • Introduction to informatics tools for data interpretation.

Main Results:

  • Detailed review of glycoproteomics origins and evolution.
  • Exemplar analyses of human glycodelin and Unio elongatulus gp273 O-glycans.
  • Introduction to GlycoWorkBench for automated data annotation.

Conclusions:

  • Glycoproteomics has advanced significantly since the 1970s.
  • Current analytical methods and tools facilitate complex glycoproteomic studies.
  • Informatics solutions like GlycoWorkBench are crucial for data interpretation.