Anti-CD25 antibodies decrease the ability of human dendritic cells to prime allogeneic CD4 T cells

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Generation of Human Monocyte-derived Dendritic Cells from Whole Blood
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K Mnasria1, C Lagaraine, F Velge-Roussel

  • 1JE 2448, Faculté de Médecine, Université François Rabelais, Tours, France.

Transplantation Proceedings
|March 31, 2009
PubMed

Anti-CD25 monoclonal antibodies are largely used in clinical transplantation to prevent acute allograft rejection episodes. Although their effects on T lymphocytes have been extensively studied, their impact on human dendritic cells (DCs) has been less reported. Furthermore, the role of the interleukin-2 in DC functions has not yet been fully elucidated. In this study, we observed that stimulation of human monocyte-derived DCs with lipopolysa ccharide or CD40L strongly induced the expression of CD25. We showed that pretreatment of DC with anti-CD25 diminished their ability to prime T-helper cells. In contrast, humanized anti-CD25 monoclonal antibodies did not affect the up-regulation of CD86, CD80, CD83, HLA-DR, or CD40 induced by lipopolysaccharide stimulation. This study supported previously unrecognized effects of anti-CD25 monoclonal antibodies on DCs that may contribute to their clinical efficacy.

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