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Hollow-fiber membrane chamber as a device for in situ environmental cultivation

Yoshiteru Aoi1, Tomoyuki Kinoshita, Toru Hata

  • 1Waseda Institute for Advanced Study, Waseda University, Shinjuku-ku, Tokyo, Japan. yoshiteruaoi@aoni.waseda.jp

Applied and Environmental Microbiology
|March 31, 2009
PubMed

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The cultivation of environmental microorganisms has long been hindered by the inability to replicate complex native conditions in vitro. The isolation chip (iChip) addresses this limitation by facilitating the growth of previously uncultivable microorganisms through in situ incubation. Designed for high-throughput microbial cultivation, the iChip comprises hundreds of microchambers, each capable of housing a single microbial cell. These microchambers are loaded with a mixture of molten agar and...