Related Experiment Video
Updated: Jun 24, 2026
Measurement of Vacuolar and Cytosolic pH In Vivo in Yeast Cell Suspensions
Published on: April 19, 2013
Yeast vitality determination based on intracellular NAD(P)H fluorescence measurement during aerobic-anaerobic
M Kurec1, G Kuncová, T Brányik
1Department of Fermentation Chemistry and Bioengineering, Institute of Chemical Technology, Prague, Czech Republic.
Abstract:
This work presents a yeast-cell vitality-assessment method based on on-line intracellular fluorescence measurement. The intracellular NAD(P)H fluorescence of a cell suspension is recorded during transition from aerobic to anaerobic conditions and the output signal is evaluated as a measure of yeast vitality (quality). This fluorescence method showed a highly satisfactory correlation with even low dead cell numbers where the acidification power test could not be applied.
