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Enterovirulent E. coli in inflammatory and noninflammatory bowel diseases

K Curová1, M Kmetová, M Sabol

  • 1Institute of Medical Microbiology and Clinical Microbiology, Pavol Jozef Safárik University in Kosice, Slovakia.

Folia Microbiologica
|March 31, 2009
PubMed

Insights

Enterovirulent E. coli (EVEC) were studied in inflammatory bowel disease (IBD) patients. Cell detachment E. coli (CDEC) were more prevalent in ulcerative colitis (UC) than Crohn

Area of Science:

  • Microbiology
  • Gastroenterology
  • Infectious Diseases

Background:

  • Inflammatory bowel disease (IBD), encompassing Crohn's disease (CD) and ulcerative colitis (UC), is associated with complex etiologies.
  • The role of specific bacterial pathotypes, such as enterovirulent E. coli (EVEC), in IBD pathogenesis requires further elucidation.

Purpose of the Study:

  • To determine the incidence and distribution of various enterovirulent E. coli (EVEC) pathotypes in E. coli strains isolated from patients with IBD (CD and UC) and non-IBD controls.
  • To investigate the potential presence of adherent-invasive E. coli (AIEC) among the isolated strains.

Main Methods:

  • Bacterial strains isolated from IBD and non-IBD patients were characterized for the presence of EVEC pathotypes.
  • Specific pathotypes including cell detachment E. coli (CDEC), enteroaggregative E. coli (EAggEC), enteropathogenic E. coli (EPEC), and enterotoxigenic E. coli (ETEC) were identified.
  • Invasion assays (gentamicin-protection assay) were performed, and genetic markers (fimA, ipaH, ial) were analyzed to assess potential AIEC strains.

Main Results:

  • Cell detachment E. coli (CDEC) were detected in 14% of all strains, with a significantly higher prevalence in UC (24.1%) compared to non-IBD (11.9%) and CD (4.7%) groups.
  • Enteroaggregative E. coli (EAggEC) (2.5%), EPEC (1.3%), and ETEC (1.5%) were also detected.
  • Enteroinvasive E. coli (EIEC) and shigatoxin-producing E. coli (STEC) were not found.
  • Some strains exhibited high invasion levels and possessed the fimA gene but lacked ipaH and ial, suggesting potential AIEC characteristics.

Conclusions:

  • CDEC exhibits a differential distribution in IBD patients, being more common in UC than CD.
  • The presence of specific EVEC strains and potentially AIEC warrants further investigation into their role in IBD pathogenesis.
  • Further comprehensive testing is required for definitive AIEC classification of the identified high-invasion strains.

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