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Updated: Jun 24, 2026
Induction of Periodontitis via a Combination of Ligature and Lipopolysaccharide Injection in a Rat Model
Published on: February 17, 2023
Myeloperoxidase as inflammatory marker of periodontal disease: experimental study in rats
Debora A S Gomes1, Juliana R Pires, Elizangela P Zuza
1Department of Diagnosis and Oral Surgery, State University of Sao Paulo, Araraquara, Sao Paulo, Brazil.
Rationale:
Previous studies have used myeloperoxidase (MPO) as an inflammatory marker to estimate the accumulation of neutrophils in inflamed regions.
Objective:
The aim of this experimental study was to quantify the levels of MPO related to experimental periodontal disease in rats.
Methods:
Periodontal disease was induced in a group of rats using placement of a ligature around molar teeth. A group of rats without ligature placement served as a control. Measurements were made on the 3(rd), 7(th), 15(th) and 30(th) day from baseline. Gingival tissues were taken for quantification of MPO levels by ELISA.
Results:
The rats with induced periodontal disease showed statistically higher MPO levels (p < 0.05) when compared to control rats. A significant increase in the levels of MPO released on days 7 and 30 was observed, with higher levels in the group with induced periodontitis.
Conclusion:
The levels of MPO were found to be higher in rats with induced periodontal disease, confirming the hypothesis that MPO may serve as an inflammatory marker for periodontitis.
