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Mesenchymal Stem Cells

Mesenchymal Stem Cells

Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their access...

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Related Experiment Video

Updated: Jun 24, 2026

Isolating Mesangiogenic Progenitor Cells (MPCs) from Human Bone Marrow

Isolating Mesangiogenic Progenitor Cells (MPCs) from Human Bone Marrow

Published on: July 15, 2016

Selective culture of mesodermal progenitor cells.

Luisa Trombi¹, Simone Pacini, Marina Montali

¹Hematology Division, Department of Oncology, Transplants, and New Advances in Medicine, University of Pisa, Via Roma 55, Pisa, Italy.

Stem Cells and Development

|April 1, 2009

Summary

Researchers identified mesodermal progenitor cells (MPCs) from bone marrow. A simple method isolates these cells, which can differentiate into various cell types, aiding stem cell research.

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Published on: July 15, 2016

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Area of Science:

Stem Cell Biology
Regenerative Medicine
Cellular Biology

Background:

Mesodermal progenitor cells (MPCs) with embryonic markers and aldehyde dehydrogenase (ALDH) activity have been identified in adult human bone marrow.
These resting MPCs can be cultured in adult human serum and easily induced to differentiate into mesenchymal stromal cells (MSCs).

Purpose of the Study:

To present a simple, inexpensive, repeatable, and standardized method for isolating highly selected MPCs from bone marrow or cord blood.
To facilitate further research on MPCs by optimizing isolation and culture conditions.

Main Methods:

Isolation of MPCs from adult human bone marrow or cord blood.
Culture optimization using commercial human AB sera and specific cell seeding concentrations.
Induction of differentiation into MSCs using fetal bovine serum (FBS) or human cord serum.

Main Results:

A pure population of MPCs can be obtained after a short culture period using optimized conditions.
MPCs differentiate into mature mesenchymal stromal cells (MSCs).
MPC-derived MSCs differentiate into osteoblasts, chondrocytes, adipocytes, and endothelial cells.

Conclusions:

A simple and cost-effective method for isolating pure MPCs has been developed.
This standardized method will significantly aid future studies on MPCs and their differentiation potential.
MPCs represent a promising cell source for regenerative medicine applications.