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The prevalence of Th17 cells in patients with dilated cardiomyopathy

An Yi1, Li Jian, Hu Xiaojing

  • 1Department of Cardiology, Affiliated Hospital, Medical College, Qingdao University, Qingdao, China. anyi1201@yahoo.cn

Clinical and Investigative Medicine. Medecine Clinique Et Experimentale
|April 1, 2009
PubMed

Insights

This study found increased T helper 17 (Th17) cells and related cytokines in patients with dilated cardiomyopathy (DCM). These findings suggest Th17 cells play a role in DCM development.

Area of Science:

  • Immunology
  • Cardiology
  • Autoimmune Diseases

Background:

  • Dilated cardiomyopathy (DCM) is a chronic autoimmune disease.
  • T helper 17 (Th17) cells are critical regulators of autoimmune and inflammatory responses.

Purpose of the Study:

  • To investigate the potential involvement of Th17 cells in the pathogenesis of DCM.
  • To compare Th17 cell frequencies and related cytokine levels in DCM patients versus healthy individuals.

Main Methods:

  • Flow cytometry was used to quantify Th17 cell frequencies in peripheral blood.
  • Enzyme-linked immunosorbent assay (ELISA) measured cytokine secretion (IL-17, IL-6, IL-23).
  • Real-time PCR assessed the expression of the transcription factor RORgammat.

Main Results:

  • DCM patients exhibited significantly higher peripheral Th17 cell frequencies compared to healthy blood donors (HBD).
  • Elevated levels of IL-17, IL-6, and IL-23 cytokines were observed in DCM patients.
  • Increased RORgammat expression was also noted in DCM patients, alongside a distinct sex-based cytokine profile.

Conclusions:

  • Th17 cell activity is enhanced in individuals with DCM.
  • These findings indicate a significant role for Th17 cells in the development of DCM.
Abstract

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