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Updated: Jun 24, 2026
Breeding by Design for Functional Rice with Genome Editing Technologies
Published on: January 3, 2025
Improved genetic mapping of endosperm traits using NCIII and TTC designs
Xuefeng Wang1, Wen Song, Zefeng Yang
1Jiangsu Provincial Key Laboratory of Crop Genetics and Physiology, Key Laboratory of Plant Functional Genomics of Ministry of Education, Yangzhou University, Yangzhou, China.
Abstract:
The endosperm in plants is a major source of human nutrition and industrial raw material. The genetic study of endosperm poses a great challenge due to its complex genetic composition and unique physical and developmental properties. In this note, we shall revisit 2 classic mating designs-North Carolina Design III (NCIII) and triple test cross (TTC)-and demonstrate their efficiency in detecting quantitative trait loci underlying endosperm traits.
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02:27Trihybrid Crosses
Some of Mendel’s crosses examined three pairs of contrasting characteristics. Such a cross is called a trihybrid cross. A trihybrid cross is a combination of three individual monohybrid crosses. For example, plant height (tall vs. short), seed shape (round vs. wrinkled), and seed color (yellow vs. green).
The F1 generation plants of a trihybrid cross are heterozygous for all three traits and produce eight gametes. Upon self-fertilization, these gametes have an equal chance to...
01:18Dihybrid Crosses