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Related Concept Videos

Single-Strand DNA Binding Proteins01:03

Single-Strand DNA Binding Proteins

For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...
Sanger Sequencing01:57

Sanger Sequencing

DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...
Southern Blot02:57

Southern Blot

Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...

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Related Experiment Video

Updated: Jun 24, 2026

A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons
11:40

A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons

Published on: November 14, 2018

Development of a sensitive deaminated single-strand conformation polymorphism (DSSCP).

Sadeq Vallian1, Isar Nassiri

  • 1Division of Genetics, Department of Biology, Faculty of Science, The University of Isfahan, Hezarjerib St., Isfahan, IR, Iran. svallian@medinews.com

Applied Biochemistry and Biotechnology
|April 1, 2009
PubMed
Summary

We developed deaminated single-strand conformation polymorphism (DSSCP), a modified DNA mutation detection method. This technique effectively identifies single nucleotide substitutions in the beta-globin gene without radioactivity.

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Last Updated: Jun 24, 2026

A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons
11:40

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Published on: November 14, 2018

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06:51

Parallel High Throughput Single Molecule Kinetic Assay for Site-Specific DNA Cleavage

Published on: May 6, 2020

Using Next Generation Sequencing to Identify Mutations Associated with Repair of a CAS9-induced Double Strand Break Near the CD4 Promoter
06:59

Using Next Generation Sequencing to Identify Mutations Associated with Repair of a CAS9-induced Double Strand Break Near the CD4 Promoter

Published on: March 31, 2022

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Single-strand conformation polymorphism (SSCP) is a common method for DNA mutation detection.
  • Sequencing complements SSCP for accurate mutation identification.
  • Limitations exist in current mutation detection methods.

Purpose of the Study:

  • To develop a modified SSCP method for enhanced DNA mutation analysis.
  • To improve the efficiency and interpretability of mutation detection.
  • To apply the novel method to identify mutations in the beta-globin gene.

Main Methods:

  • Modified SSCP using sodium bisulfite treatment (deaminated SSCP or DSSCP).
  • Polymerase chain reaction (PCR) amplification of beta-globin gene exon 3.
  • Analysis of PCR products on non-denaturing polyacrylamide gels.
  • Sequencing to confirm identified mutations.

Main Results:

  • Successfully applied DSSCP to analyze mutations in the beta-globin gene.
  • Identified homozygote and heterozygote single nucleotide substitutions.
  • DSSCP provided clear, reproducible results.
  • The method does not require radioactivity.

Conclusions:

  • DSSCP is an effective and reproducible method for DNA mutation detection.
  • The modified SSCP offers advantages in clarity and ease of interpretation.
  • DSSCP is a valuable tool for analyzing mutations, particularly in the beta-globin gene.