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Published on: July 11, 2015
Occurrence of false-positive results in three paratuberculosis - ELISAs performed in a tuberculous herd
W Lilenbaum1, C D Marassi, R Varges
1Veterinary Bacteriology Laboratory, Universidade Federal Fluminense, Niteroi, 24210-130, Brazil. mipwalt@vm.uff.br
Abstract:
The interference of bovine tuberculosis (TB) on the efficacy of paratuberculosis (PTB) diagnostic tests has been evaluated. A group of 32 tuberculous cows identified by both intradermal tests and gamma-interferon assay, 16 of them confirmed by the recovery of M.bovis from tissues, was tested by three different PTB- ELISAs, being two commercials and one in-house. The rest of the adult animals of the herds, totalizing 216 TB-negative animals, were also tested as a control group. Fecal culture for PTB was negative in all animals, but seven (21.8%) tuberculous cows produced false-positive reactions when tested by various PTB-ELISAs, leading to a misdiagnosis. Tuberculosis impairs the specificity of serological tests for paratuberculosis diagnosis and should be considered for the reliability of PTB control programs.
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