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Updated: Jun 24, 2026
12:11
Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization
Published on: February 27, 2020
Semi-automated tandem mass spectrometric (MS/MS) triple quadrupole operating parameter optimization for
Kristin Geddes1, Gary Adamson, Neal Dube
1Drug Metabolism and Pharmacokinetics, Merck & Co., Inc., Sumneytown Pike, West Point, PA, USA.
Rapid Communications in Mass Spectrometry : RCM
|April 1, 2009
Summary
This study presents an automated workflow for optimizing mass spectrometric (MS) instrument parameters and selected reaction monitoring (SRM) transitions, improving method development efficiency.
Area of Science:
- Analytical Chemistry
- Biochemistry
Background:
- Optimizing mass spectrometric (MS) instrument parameters and selected reaction monitoring (SRM) transitions is crucial for accurate quantitative analysis.
- Traditional methods for optimization can be time-consuming and labor-intensive.
Purpose of the Study:
- To describe an automated workflow for determining SRM transitions and optimum MS instrument parameters.
- To compare the efficiency of this automated approach with traditional optimization methods.
Main Methods:
- Utilized a Nanomate for automated sample infusion.
- Employed Automaton optimization software for parameter determination.
- Stored results in Analyst software Compound Database for automated acquisition method building.
Main Results:
- The Nanomate-Automaton approach demonstrated efficiency in optimizing SRM transitions and MS parameters.
- Acquisition methods developed on Sciex API3000 were successfully transferred to API4000 and API5000 instruments.
Conclusions:
- The described automated workflow streamlines the optimization process for SRM assays.
- This automated method facilitates robust and reproducible quantitative analysis using mass spectrometry.
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