[An experimental study on the cytokine expression of macrophage influenced by biomaterials]

This experiment was designed to investigate the influence of two biomaterials, titanium oxide (Ti-O) and stainless steel (SS), on the cytokine expression of macrophage, and further, to evaluate their biocompatibility. After being co-cultured with Ti-O and SS for 72 h, the cell number and morphology of macrophages attached on materials were detected by fluorescent microscope and SEM. Nitride oxide (NO) and monocyte chemoattractant protein 1 (MCP-1) released by the macrophages co-cultured with different materials were also examined and compared. We found that the cell number of macrophages attached to Ti-O was smaller than that attached to SS. The levels of NO and MCP-1 released by the macrophages co-cultured with Ti-O were lower when compared with those released by macrophages co-cultured with SS. These results demonstrate that Ti-O has better biocompatibility than does SS.