Related Experiment Video
Updated: Jun 24, 2026
A Technique to Functionalize and Self-assemble Macroscopic Nanoparticle-ligand Monolayer Films onto Template-free Substrates
Published on: May 9, 2014
Layer-by-layer assembly of conjugated polyelectrolytes on magnetic nanoparticle surfaces
Bin Sun1, Yang Zhang, Ke-Jun Gu
1Department of Polymer Science & Engineering and Key Laboratory of Mesoscopic Chemistry of MOE, School of Chemistry & Chemical Engineering, Nanjing University, Nanjing 210093, China.
Abstract:
Composite nanoparticles with magnetic core and fluorescent shell were facilely prepared by the layer-by-layer deposition of conjugated polyelectrolytes over the negatively charged nanoparticles (NPs) of superparamagnetic iron oxide. The alternate assembly of cationic and anionic fluorescent polyelectrolytes leads to reversal in the sign of zeta-potentials. The even numbers of adsorption layer corresponding to the anionic polyelectrolyte (PFS) have negative values (-13 to -24 mV), whereas odd numbers of coating relative to the cationic polyelectrolyte (PFN) have positive values (26 to 28 mV). The composite nanoparticles can respond to both external magnetic field and ultraviolet light excitation. Forster resonance energy transfer (FRET) between oppositely charged polyelectrolytes (PFN and ThPFS) layers was also found, indicating dense packing of the polymer coatings. The fluorescence of the positively charged nanoparticles (NPs/PFN) can be quenched with very high efficiency by a small molecule anionic quencher [Fe(CN)6(4-)], while the same quencher has far less effect on the fluorescence of the negatively charged nanoparticles (NPs/PFN/PFS).
