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A Straightforward Method for Glucosinolate Extraction and Analysis with High-pressure Liquid Chromatography (HPLC)
Published on: March 15, 2017
Updated glucosinolate profile of Dithyrea wislizenii
Sabine Montaut1, Julie Grandbois, Laura Righetti
1Department of Chemistry & Biochemistry, Laurentian University, 935 Ramsey Lake Road, Sudbury, ON P3E 2C6, Canada. smontaut@laurentian.ca
Journal of Natural Products
|April 2, 2009
Summary
This study identified glucosinolates in Dithyrea wislizenii fruit extracts, including sinigrin and four novel compounds. These findings contribute to understanding the chemical composition of this plant species.
Area of Science:
- Plant biochemistry and metabolomics
- Natural product chemistry
Background:
- Dithyrea wislizenii is a plant species with potential for novel compound discovery.
- Glucosinolates are sulfur- and nitrogen-containing compounds found in plants, known for their diverse biological activities.
Purpose of the Study:
- To analyze the glucosinolate profile of Dithyrea wislizenii fruit extracts.
- To identify and characterize intact glucosinolates and desulfoglucosinolates present in the plant.
Main Methods:
- High-Performance Liquid Chromatography coupled with Atmospheric Pressure Chemical Ionization Mass Spectrometry (HPLC-APCI-MS) for desulfoglucosinolates.
- High-Performance Liquid Chromatography coupled with Electrospray Ionization Mass Spectrometry (HPLC-ESI-MS) for intact glucosinolates.
- Nuclear Magnetic Resonance (NMR) and Mass Spectrometry (MS) for structural elucidation.
Main Results:
- The presence of 2-propenylglucosinolate (sinigrin) was confirmed.
- Four novel glucosinolates were isolated and characterized: 6-methylsulfanylhexyl- (glucolesquerellin 9), 6-methylsulfinylhexyl- (glucohesperin 10), 7-methylsulfanylheptyl- (11), and 5-methylsulfanylpentylglucosinolate (glucoberteroin 12).
- 7-Methoxyglucobrassicin was not detected in the analyzed extracts.
Conclusions:
- The study successfully identified a range of glucosinolates in Dithyrea wislizenii, including previously undescribed compounds.
- The findings expand the knowledge of glucosinolate diversity in Brassicaceae family plants.
