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Related Concept Videos

Preparation of Samples for Electron Microscopy01:20

Preparation of Samples for Electron Microscopy

To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does not destroy them and dried thoroughly (desiccated/dehydrated) so the vacuum does not affect them. Fixation needs to be done as quickly as possible because the sample properties will start changing as soon as it is removed from its natural environment. For example, in a tissue sample, the oxygen levels begin decreasing, causing an altered...
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Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.
Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...

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Related Experiment Video

Updated: Jun 24, 2026

Fabrication of Zero Mode Waveguides for High Concentration Single Molecule Microscopy
08:01

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Published on: May 12, 2020

Etching gold tips suitable for tip-enhanced near-field optical microscopy.

Lior Eligal1, Ferhat Culfaz, Vincent McCaughan

  • 1Department of Physics, King's College London, Strand, London WC2R 2LS, United Kingdom.

The Review of Scientific Instruments
|April 2, 2009
PubMed
Summary

Researchers developed a fast, single-step electrochemical etching method to create high-quality gold tips for tip-enhanced near-field optical microscopy. These reproducible tips enable detailed imaging of single quantum dots.

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Last Updated: Jun 24, 2026

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Area of Science:

  • Materials Science
  • Nanotechnology
  • Optical Microscopy

Background:

  • Tip-enhanced near-field optical microscopy (TENORM) requires specialized probes.
  • Fabricating high-quality, reproducible tips for TENORM is challenging and time-consuming.

Purpose of the Study:

  • To develop a simple, rapid, and reproducible method for fabricating gold tips for TENORM.
  • To characterize the fabricated tips and demonstrate their utility in imaging.

Main Methods:

  • A single-step direct current electrochemical etch was employed to fabricate gold tips.
  • The etching process was analyzed in detail to ensure reproducibility.
  • Tip geometry, including radius of curvature and aspect ratio, was optimized.

Main Results:

  • Smooth gold tips with a radius of curvature of approximately 40 nm were produced within minutes.
  • The tips exhibited an aspect ratio suitable for shear force measurements.
  • Reproducible, high-quality tips were consistently fabricated.
  • Near-field images of single quantum dots were successfully obtained using the fabricated tips.

Conclusions:

  • The described electrochemical etching method offers a simple and efficient way to produce gold tips for TENORM.
  • This technique enables the generation of reproducible, high-quality tips essential for advanced optical microscopy.
  • The method facilitates high-resolution imaging of nanoscale phenomena, such as single quantum dots.