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Updated: Jun 24, 2026

Detection of Viral RNA by Fluorescence in situ Hybridization (FISH)
10:16

Detection of Viral RNA by Fluorescence in situ Hybridization (FISH)

Published on: May 5, 2012

Identification of LTR-specific small non-coding RNA in FeLV infected cells

Lora W Forman1, Ruma Pal-Ghosh, Remco A Spanjaard

  • 1Cancer Research Center, Department of Medicine, Boston University School of Medicine, 72 East Concord Street, Boston, MA 02458, USA.

FEBS Letters
|April 2, 2009
PubMed

Insights

Researchers discovered a novel small non-coding RNA from the feline leukemia virus (FeLV) long terminal repeat (LTR) region. This LTR-RNA activates NF-kappaB signaling, suggesting a role in FeLV pathogenesis.

Area of Science:

  • Virology
  • Molecular Biology
  • Oncology

Background:

  • The U3-LTR region of leukemia viruses is known to activate cancer-related signaling pathways.
  • The precise role of non-coding RNA transcripts produced by these regions during viral infection is not fully understood.

Purpose of the Study:

  • To investigate the production and function of LTR-specific small non-coding RNAs in feline leukemia virus (FeLV) infection.
  • To determine the potential role of these transcripts in FeLV pathogenesis.

Main Methods:

  • Utilized a feline cell line infected with FeLV.
  • Employed RNA cloning techniques to identify and characterize small non-coding RNAs originating from the U3-LTR region.
  • Performed in vitro assays to assess the signaling pathway activation by the identified LTR-RNA transcript.

Main Results:

  • Successfully identified and cloned a novel 104-base small non-coding RNA transcript originating from the FeLV U3-LTR region.
  • Demonstrated that this LTR-RNA transcript activates the NF-kappaB signaling pathway in in vitro assays.

Conclusions:

  • This study provides the first evidence of an LTR-specific small non-coding RNA in FeLV-infected cells.
  • The findings suggest a potential role for this LTR-RNA transcript in the pathogenesis of FeLV by activating NF-kappaB signaling.

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