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Published on: November 13, 2017
Rapid antibody selection by mRNA display on a microfluidic chip.
Noriko Tabata1, Yuko Sakuma, Yumiko Honda
1Department of Biosciences and Informatics, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama 223-8522, Japan.
Nucleic Acids Research
|April 2, 2009
Summary
This study combines mRNA display and microfluidics for rapid antibody selection, achieving high-affinity antibodies in one to two rounds. This accelerates antibody discovery and protein interaction identification for therapeutic applications.
Area of Science:
- Biotechnology
- Molecular Biology
- Immunology
Background:
- In vitro antibody display technologies are crucial for isolating monoclonal antibodies.
- Current methods require multiple rounds of affinity selection, which is time-consuming.
Purpose of the Study:
- To develop a faster and more efficient method for antibody selection and evolution.
- To achieve ultrahigh enrichment efficiency in antibody discovery.
Main Methods:
- Combination of mRNA display with a microfluidic system.
- Selection and evolution of antibodies from naive and randomized single-chain variable fragment (scFv) libraries.
Main Results:
- Achieved ultrahigh enrichment efficiency of 10(6)- to 10(8)-fold per round.
- Obtained high-affinity and specific antibodies after one or two selection rounds from libraries of ~10(12) molecules.
- Successfully selected protein-protein, protein-DNA, and protein-drug interactions with high efficiency.
Conclusions:
- The integrated mRNA display and microfluidic system significantly accelerates antibody preparation.
- This method facilitates high-throughput identification of protein interactions, benefiting proteomic and therapeutic research.
