Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Fluorescence and Phosphorescence: Instrumentation01:25

Fluorescence and Phosphorescence: Instrumentation

Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Spectral dependence of lipofuscin fluorescence lifetimes revealed by FLIM with a superconducting nanowire single-photon detector.

Journal of biomedical optics·2026
Same author

Integrating cross-omics research through FAIR Digital Objects with DataPLANT.

Journal of integrative bioinformatics·2026
Same author

The developing leaf of the wild grass Brachypodium distachyon at single-cell resolution.

The Plant cell·2026
Same author

In situ architecture of plasmodesmata in Physcomitrium patens resolved by cryo-electron tomography.

Nature plants·2026
Same author

Usage of a split CH2 domain as building block for new antibody formats.

Protein engineering, design & selection : PEDS·2026
Same author

The transcription factor NO TRANSMITTING TRACT/WIP2 modulates cytokinin homeostasis in Arabidopsis.

The Plant journal : for cell and molecular biology·2026
Same journal

Synthesis of tetrazole based donor-acceptor type fluorescent molecules and their interaction with transport protein bovine serum albumin.

Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology·2026
Same journal

Rational design of an S-scheme 2D/3D ZnIn<sub>2</sub>S<sub>4</sub>/K<sub>3</sub>PW<sub>12</sub>O<sub>40</sub> heterojunction for enhanced photocatalytic antibiotic degradation.

Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology·2026
Same journal

Chlorin e6-mediated sublethal photodynamic therapy modulates biomarkers in a murine lung cancer-derived in vitro cancer-associated fibroblast model.

Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology·2026
Same journal

Polyethyleneimine-protoporphyrin IX and its Zn(II) complex conjugates for photodynamic inactivation of Staphylococcus aureus on cucumber mesocarp surfaces.

Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology·2026
Same journal

Canopy chlorophyll fluorescence escape fraction under polychromatic irradiation.

Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology·2026
Same journal

Genomic analysis highlights a novel class of DPCF-type cyanobacteriochrome.

Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology·2026
See all related articles

Related Experiment Video

Updated: Jun 24, 2026

Using Three-color Single-molecule FRET to Study the Correlation of Protein Interactions
11:22

Using Three-color Single-molecule FRET to Study the Correlation of Protein Interactions

Published on: January 30, 2018

Multiparameter fluorescence image spectroscopy to study molecular interactions.

Stefanie Weidtkamp-Peters1, Suren Felekyan, Andrea Bleckmann

  • 1Lehrstuhl für Molekulare Physikalische Chemie, Heinrich-Heine-Universität Düsseldorf, Universitätsstr. 1, 40225, Düsseldorf, Germany. stefanie.weidtkamp-peters@uni-duesseldorf.de

Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology
|April 2, 2009
PubMed
Summary
This summary is machine-generated.

Multiparameter Fluorescence Image Spectroscopy (MFIS) enables simultaneous monitoring of fluorescence parameters. This method enhances selectivity and detection of heterogeneities in complex biological systems.

More Related Videos

High Precision FRET at Single-molecule Level for Biomolecule Structure Determination
11:24

High Precision FRET at Single-molecule Level for Biomolecule Structure Determination

Published on: May 13, 2017

Related Experiment Videos

Last Updated: Jun 24, 2026

Using Three-color Single-molecule FRET to Study the Correlation of Protein Interactions
11:22

Using Three-color Single-molecule FRET to Study the Correlation of Protein Interactions

Published on: January 30, 2018

High Precision FRET at Single-molecule Level for Biomolecule Structure Determination
11:24

High Precision FRET at Single-molecule Level for Biomolecule Structure Determination

Published on: May 13, 2017

Area of Science:

  • Biophysics
  • Cell Biology
  • Microscopy

Background:

  • Confocal fluorescence microscopy is crucial for biological research.
  • Simultaneous measurement of multiple fluorescence parameters is challenging.
  • Existing methods often lack the sensitivity to detect subtle molecular interactions.

Purpose of the Study:

  • To introduce and validate Multiparameter Fluorescence Image Spectroscopy (MFIS) for advanced microscopy.
  • To demonstrate the capability of MFIS in analyzing complex biological environments.
  • To showcase the utility of MFIS in molecular interaction studies.

Main Methods:

  • Utilizing photon-by-photon registration for parallel recording of Fluorescence Correlation Spectroscopy (FCS/FCCS), fluorescence lifetime, and image data.
  • Employing higher-dimensional histograms for analyzing pixel fluorescence information.
  • Applying MFIS to study diffusion heterogeneity, Förster Resonance Energy Transfer (FRET), and protein homodimerization.

Main Results:

  • MFIS achieved picosecond accuracy over hours-long measurements.
  • Demonstrated detection of diffusion heterogeneity in sepharose beads.
  • Successfully conducted FRET studies in mammalian and plant cells, including transcription factor dimerization.
  • Multidimensional analysis of correlated parameters (FRET, FCS, lifetime, anisotropy) significantly increased analysis robustness.

Conclusions:

  • MFIS offers enhanced selectivity and statistically relevant data analysis for detecting heterogeneities.
  • The method is reliable for molecular interaction studies in diverse and complex biological settings.
  • MFIS enables efficient detection of molecular dynamics and interactions at low expression levels, down to the single-molecule level.