Related Experiment Video
Updated: Jun 24, 2026
Screening Assays to Characterize Novel Endothelial Regulators Involved in the Inflammatory Response
Published on: September 15, 2017
Interleukin-4 induces expression of eotaxin in endometriotic stromal cells
Zhuo Ouyang1, Yutaka Osuga, Yasushi Hirota
1Department of Obstetrics and Gynecology, Faculty of Medicine, University of Tokyo, Tokyo, Japan.
Objective:
To study the relationship between eotaxin and interleukin-4 (IL-4) in the pathophysiology of endometriosis.
Design:
Comparative and laboratory study.
Setting:
University teaching hospital reproductive endocrinology and infertility practice.
Patient(S):
Ectopic endometrial tissues were collected from women with endometriosis.
Intervention(S):
Ectopic endometrial stromal cells (ESCs) were isolated and cultured with IL-4. Ectopic endometriotic tissues were immunostained for eotaxin and IL-4.
Main Outcome Measure(S):
Gene expression of eotaxin was determined by standard and real-time reverse-transcriptase polymerase chain reaction. Secretion of eotaxin from ESC was measured using specific ELISA. The immunostained sections were examined.
Result(S):
Interleukin-4 (IL-4) increased mRNA expression and protein secretion of eotaxin from ESC in a dose-dependent manner. Immunohistochemical analysis showed that eotaxin-positive cells colocalized with IL-4-positive cells and accumulated around the blood vessels in the stroma of endometriotic tissue.
Conclusion(S):
IL-4 induces eotaxin in ESCs, which might promote angiogenesis and the subsequent development of endometriosis.
More Related Videos
08:02Mouse Model of Surgically-induced Endometriosis by Auto-transplantation of Uterine Tissue
Published on: January 6, 2012
08:47Induction of Experimental Autoimmune Encephalomyelitis in Mice and Evaluation of the Disease-dependent Distribution of Immune Cells in Various Tissues
Published on: May 8, 2016
