Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 24, 2026

Optimizing Attachment of Human Mesenchymal Stem Cells on Poly(ε-caprolactone) Electrospun Yarns
10:50

Optimizing Attachment of Human Mesenchymal Stem Cells on Poly(ε-caprolactone) Electrospun Yarns

Published on: April 10, 2015

Embedding methods for poly(L-lactic acid) microfiber mesh/human mesenchymal stem cell constructs.

D D'Alessandro1, B Battolla, L Trombi

  • 1CUCCS-RRMR, Center for the Clinical Use of Stem Cells-Regional Network of Regenerative Medicine, University of Pisa, Italy.

Micron (Oxford, England : 1993)
|April 3, 2009
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effectiveness of Baricitinib in Alopecia Areata Patients With a Current Episode Lasting Longer Than 8 Years: An Italian Multicenter Real-Life Experience in the Lazio Region.

International journal of dermatology·2025
Same author

Safe Integration of Encorafenib plus Binimetinib with Stereotactic Radiosurgery for Melanoma Brain Metastases: a Case Report.

La Clinica terapeutica·2024
Same author

Correction: <i>Lactiplantibacillus plantarum</i> HEAL9 attenuates cognitive impairment and progression of Alzheimer's disease and related bowel symptoms in SAMP8 mice by modulating microbiota-gut-inflammasome-brain axis.

Food & function·2024
Same author

Eczematous reactions in patients with plaque psoriasis receiving biological therapy: an observational study.

European review for medical and pharmacological sciences·2024
Same author

<i>Lactiplantibacillus plantarum</i> HEAL9 attenuates cognitive impairment and progression of Alzheimer's disease and related bowel symptoms in SAMP8 mice by modulating microbiota-gut-inflammasome-brain axis.

Food & function·2024
Same author

Efficacy and Safety of bimekizumab in elderly patients: real-world multicenter retrospective study - IL PSO (Italian Landscape Psoriasis).

The Journal of dermatological treatment·2024
Same journal

Immunofluorescence study and morphometric analysis of collagen-IV in the exchange tissue of the quail (Coturnix coturnix) lung by confocal laser scanning microscopy.

Micron (Oxford, England : 1993)·2026
Same journal

Micromorphological study of leaf surfaces structures in selected Crataegus L. (Rosaceae) species using light, scanning electron, and confocal laser microscopy.

Micron (Oxford, England : 1993)·2026
Same journal

3D reconstruction of the nymphal feeding apparatus of Philaenus spumarius.

Micron (Oxford, England : 1993)·2026
Same journal

The influence of physicians and surgeons on Leeuwenhoek's observations of crystal formation.

Micron (Oxford, England : 1993)·2026
Same journal

Distribution of telocytes in the choroid (eye) of a teleost: An ultrastructural observation.

Micron (Oxford, England : 1993)·2026
Same journal

SEM-EDAX: A tool for microanalytical elemental mapping in butterfly wing scales.

Micron (Oxford, England : 1993)·2026
See all related articles

This study presents a new histological method for analyzing poly(L-lactic acid) (PLLA) microfiber scaffolds seeded with human mesenchymal stromal cells (hMSCs). The method confirms cell viability and provides insights into cell distribution and extracellular matrix production in tissue engineering constructs.

Area of Science:

  • Tissue Engineering
  • Biomaterials Science
  • Cell Biology

Background:

  • Poly(L-lactic acid) (PLLA) fiber mesh scaffolds are explored for tissue repair, supporting stem cell growth and differentiation.
  • Existing studies on PLLA microfiber/cell constructs lack detailed histological analysis, limiting understanding of cell-scaffold interactions and extracellular matrix (ECM) production.
  • Histological evaluation is crucial for assessing cellular distribution, cell-scaffold integration, and ECM synthesis within engineered tissues.

Purpose of the Study:

  • To develop and validate a robust histological and histochemical method for analyzing poly(L-lactic acid) (PLLA) microfiber/cell constructs.
  • To investigate the compatibility and behavior of bone marrow-derived human mesenchymal stromal cells (hMSCs) within PLLA microfiber scaffolds over time.

Main Methods:

More Related Videos

Use of Human Perivascular Stem Cells for Bone Regeneration
07:05

Use of Human Perivascular Stem Cells for Bone Regeneration

Published on: May 25, 2012

Related Experiment Videos

Last Updated: Jun 24, 2026

Optimizing Attachment of Human Mesenchymal Stem Cells on Poly(&#949;-caprolactone) Electrospun Yarns
10:50

Optimizing Attachment of Human Mesenchymal Stem Cells on Poly(ε-caprolactone) Electrospun Yarns

Published on: April 10, 2015

Use of Human Perivascular Stem Cells for Bone Regeneration
07:05

Use of Human Perivascular Stem Cells for Bone Regeneration

Published on: May 25, 2012

  • Biodegradable non-woven fiber meshes were fabricated using hollow PLLA microfibers.
  • Various embedding techniques (paraffin, Killik, acrylic resin) were evaluated; acrylic resin proved most suitable for histological analysis.
  • PLLA microfiber/hMSC constructs were embedded in acrylic resin and analyzed using Toluidine Blue, Periodic Acid-Schiff (PAS), and Alcian Blue staining, alongside MTT and CCK-8 assays for cell viability.

Main Results:

  • Acrylic resin embedding successfully preserved the structural integrity of PLLA microfiber/hMSC constructs for histological analysis.
  • Histochemical staining confirmed cell viability and proliferation up to 6 weeks, consistent with MTT and CCK-8 data.
  • Staining revealed detailed information on cell distribution, organization within the scaffold, and the production of ECM molecules, enriching previous SEM and quantitative findings.

Conclusions:

  • A validated histological and histochemical method using acrylic resin embedding is proposed for analyzing PLLA microfiber/cell constructs.
  • The study confirms the compatibility of PLLA microfibers with hMSCs, demonstrating sustained cell viability and ECM production.
  • This methodological advancement provides deeper insights into cell-scaffold interactions, crucial for advancing PLLA-based tissue engineering applications.