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Related Concept Videos

Mass Analyzers: Common Types01:19

Mass Analyzers: Common Types

The quadrupole mass analyzer consists of four cylindrical metal rods arranged in a diamond carrying a DC voltage and a radio-frequency AC voltage. The motion of ions through the quadrupole depends on the field strength, causing only ions of a certain m/z to resonate successfully and strike the detector at a given field strength. Though the transmission rate for these analyzers is high, the exact elemental composition of the sample is not determined because of low resolution; however, they are...
Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
Mass Spectrometers01:16

Mass Spectrometers

This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
Mass Spectrometry: Overview01:19

Mass Spectrometry: Overview

Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass. One common type of ionization, known as electron ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave behind a...
Mass Spectrum: Interpretation01:24

Mass Spectrum: Interpretation

An unknown compound can be established by identifying the molecular ion peak in the mass spectrum. The molecular ion peak is often weak or absent due to the predominance of fragmentation in high-energy electron beams. In such cases, a soft-energy electron beam can be used to scan the spectrum to enhance the intensity of the molecular ion peak. Additionally, chemical ionization, field ionization, and desorption ionization spectra are used to obtain a relatively intense molecular ion peak.To...

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Sample Preparation for Mass-spectrometry-based Proteomics Analysis of Ocular Microvessels
11:32

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Published on: February 22, 2019

Quantitative-qualitative data acquisition using a benchtop Orbitrap mass spectrometer.

Kevin P Bateman1, Markus Kellmann, Helmut Muenster

  • 1Merck Frosst Canada Ltd., Kirkland, Quebec, Canada. kevin_bateman@merck.com

Journal of the American Society for Mass Spectrometry
|April 3, 2009
PubMed
Summary
This summary is machine-generated.

This study demonstrates a new Orbitrap mass analyzer for drug metabolism studies. It enables simultaneous quantitative and qualitative analysis, improving metabolite detection without compound-specific optimization.

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Area of Science:

  • Analytical Chemistry
  • Mass Spectrometry
  • Pharmacology

Background:

  • Current drug metabolism studies rely on triple-quadrupole mass analyzers, requiring extensive parameter optimization.
  • These methods offer limited scope, detecting only specified compounds and losing valuable metabolite information.

Purpose of the Study:

  • To evaluate a prototype benchtop Orbitrap mass analyzer for simultaneous quantitative and qualitative analysis in drug discovery.
  • To assess its suitability for discovery-stage pharmacokinetic and in vitro metabolism studies.

Main Methods:

  • Utilized a prototype benchtop Orbitrap mass analyzer for full-scan acquisition.
  • Analyzed human microsomal incubation samples and rat plasma from pharmacokinetic studies.
  • Evaluated instrumental parameters including scan speed, resolution, and mass accuracy.

Main Results:

  • The Orbitrap system provided both quantitative and qualitative data from complex biological matrices.
  • Demonstrated the potential for sensitive and selective quantitative analysis.
  • Successfully characterized metabolites alongside quantitative measurements.

Conclusions:

  • A benchtop Orbitrap mass analyzer can support a quantitative-qualitative workflow for drug metabolism studies.
  • This approach eliminates the need for compound-specific optimization, enhancing efficiency.
  • Enables simultaneous detection and characterization of drug compounds and their metabolites.