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Ethanol improves the transformation efficiency of intact yeast cells
1Department of Microbiology, Charles University, Vinicna, Prague, Czechoslovakia.
Current Genetics
|July 1, 1991
Abstract:
A technique is described in which ethanol is used to improve the genetic transformation of intact yeast (Saccharomyces cerevisiae) cells pretreated with LiAc and PEG. Transformation efficiency was increased with increasing concentrations of ethanol with a peak at 10% concentration. The effect varies with different yeast strains and plasmids and up to a maximum of a 15-fold increase was observed.