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Related Experiment Videos

Subcellular localization of enzymes.

F G Zaki

    Annals of Clinical and Laboratory Science
    |May 1, 1977
    PubMed
    Summary
    This summary is machine-generated.

    High-resolution histochemistry techniques enable precise subcellular enzyme localization. Absence of glucose-6-phosphatase activity in liver cells indicates early preneoplastic changes.

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    Area of Science:

    • Biochemistry
    • Cell Biology
    • Histochemistry

    Background:

    • Subcellular enzyme localization demands high-resolution techniques.
    • Conventional light microscopic histochemistry methods are adapted for ultrastructural analysis.

    Purpose of the Study:

    • To detail modified histochemical procedures for high-resolution subcellular enzyme localization.
    • To exemplify these techniques using enzymes in hepatocytic organelles.

    Main Methods:

    • Short fixation, enzyme activity capture, postosmication, dehydration.
    • Embedding in plastic media for electron microscopy.
    • Application to hepatocytic organelles and responses to varied conditions.

    Main Results:

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  • Established procedures allow precise localization of enzyme activities within subcellular compartments.
  • Demonstrated applicability to specific hepatocytic enzymes.

    • Conclusions:

    • Modified histochemical techniques are crucial for ultrastructural enzyme mapping.
    • Loss of glucose-6-phosphatase activity serves as an early marker for preneoplastic liver cells.