Related Experiment Video
Updated: Jun 24, 2026
09:15
Combining X-Ray Crystallography with Small Angle X-Ray Scattering to Model Unstructured Regions of Nsa1 from S. Cerevisiae
Published on: January 10, 2018
Expression, purification, crystallization and preliminary X-ray crystallographic analysis of the SH3 domain of human
Zhuliang Shi1, Ning Liang, Wei Xu
1Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, People's Republic of China.
Abstract:
The SH3 domain of human AHI1 was cloned and expressed in Escherichia coli. The protein was purified by affinity and size-exclusion chromatography and was crystallized using the sitting-drop vapour-diffusion method at 293 K. A complete data set was collected to 2.5 A resolution at 110 K. The crystal belonged to space group P4(1)2(1)2, with unit-cell parameters a = 67.377, b = 67.377, c = 98.549 A.
