Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Voltammetric Techniques: Cyclic Voltammetry

Voltammetric Techniques: Cyclic Voltammetry

Cyclic voltammetry (CV) is an electrochemical technique used to investigate the redox properties of a chemical species. It involves measuring the current response of an electrochemical cell as a function of the applied potential. The setup for cyclic voltammetry typically consists of a working electrode, a reference electrode, and a counter electrode—all immersed in an electrolyte solution. The working electrode is where the redox reaction of interest occurs, while the reference electrode...

Voltammetric Techniques: Pulse Voltammetry

Voltammetric Techniques: Pulse Voltammetry

Differential-pulse voltammetry (DPV) is a type of voltammetry that involves applying a series of voltage pulses to an electrochemical cell while measuring the resulting current. In DPV, the differential pulse or small potential pulses are superimposed on a linear potential sweep. The magnitude of these pulses is typically small, often in the millivolt range. Each voltage pulse lasts a short duration, usually in the order of a few milliseconds, and is applied at regular intervals along the...

Voltammetry: Factors Affecting Measurements

Voltammetry: Factors Affecting Measurements

A current produced due to the redox reactions of the analyte at the working and auxiliary electrodes is called a faradaic current. The reaction can be divided into two types. The current generated due to the reduction of the analyte is called cathodic current, and it carries a positive charge. In contrast, the current produced by analyte oxidation is known as an anodic current, and it has a negative charge. The applied potential at the working electrode determines the faradaic current flow, and...

Voltammetric Techniques: Linear-Scan (E vs Time)

Voltammetric Techniques: Linear-Scan (E vs Time)

Polarography is a classical voltammetric technique used to analyze electrochemical reactions. This method applies a linear potential sweep to a dropping mercury electrode (DME), and the resulting current is measured. A dropping mercury electrode is commonly used as the working electrode in polarography. It consists of a capillary tube filled with mercury, where the tiny droplet forms at the tip. This droplet continuously drops from the capillary, creating a new electrode surface for each...

Voltammetry: Stripping Methods

Voltammetry: Stripping Methods

Anodic Stripping Voltammetry (ASV), Cathodic Stripping Voltammetry (CSV), and Adsorptive Stripping Voltammetry (AdSV) are electrochemical techniques used to determine trace amounts of analytes in solution. These methods involve applying a potential to an electrode and measuring the resulting current.
Anodic Stripping Voltammetry (ASV)
ASV is used to determine metals and metalloids at trace levels. It involves two steps: deposition and stripping. First, a negative potential is applied to the...

Voltammetry: Overview

Voltammetry: Overview

Voltammetry is an electroanalytical technique in which the current flowing through an electrochemical cell is measured as a function of applied potential, typically under conditions of concentration polarization. The technique provides valuable information about redox-active species, and the current response is plotted as a voltammogram.
A voltammetric cell uses three electrodes: a working electrode, a reference electrode, and an auxiliary electrode. The redox reactions occur in the working...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

3D Bioprinting of Blood Vessel Model for Improving Wound Healing.

International journal of molecular sciences·2026

Same author

"The future depends on what we do in the present" - Development positions of EU countries by levels of sustainable development and living standards.

PloS one·2025

Same author

Development of Functional Foods: A Comparative Study on the Polyphenols and Anthocyanins Content in Chokeberry and Blueberry Pomace Extracts and Their Antitumor Properties.

Foods (Basel, Switzerland)·2024

Same author

PCR Array Profiling of miRNA Expression Involved in the Differentiation of Amniotic Fluid Stem Cells toward Endothelial and Smooth Muscle Progenitor Cells.

International journal of molecular sciences·2024

Same author

Comprehensive and critical view on the anti-inflammatory and immunomodulatory role of natural phenolic antioxidants.

European journal of medicinal chemistry·2023

Same author

A Multi-Pumping Gradient Calibration Module for Potentiometric Determination of Nitrate in Surface Water.

Molecules (Basel, Switzerland)·2023

Search research articles

Related Experiment Video

Updated: Jun 24, 2026

A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells

A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells

Published on: April 11, 2014

Ascorbic Acid determination in commercial fruit juice samples by cyclic voltammetry.

Aurelia Magdalena Pisoschi¹, Andrei Florin Danet, Slawomir Kalinowski

¹Chemistry and Biochemistry Department, Faculty of Veterinary Medicine, University of Agronomic Sciences and Veterinary Medicine-Bucharest, Bulevardul Marasti, no 59, 011464 Bucharest, Romania.

Journal of Automated Methods & Management in Chemistry

|April 4, 2009

Summary

A new cyclic voltammetry method accurately measures ascorbic acid in fruit juices. This electrochemical technique provides reliable results comparable to traditional methods, ensuring quality control for vitamin C content.

More Related Videos

Improved UPLC-UV Method for the Quantification of Vitamin C in Lettuce Varieties (Lactuca sativa L.) and Crop Wild Relatives (Lactuca spp.)

Improved UPLC-UV Method for the Quantification of Vitamin C in Lettuce Varieties (Lactuca sativa L.) and Crop Wild Relatives (Lactuca spp.)

Published on: June 30, 2020

Examination of Rapid Dopamine Dynamics with Fast Scan Cyclic Voltammetry During Intra-oral Tastant Administration in Awake Rats

Examination of Rapid Dopamine Dynamics with Fast Scan Cyclic Voltammetry During Intra-oral Tastant Administration in Awake Rats

Published on: August 12, 2015

Related Experiment Videos

Last Updated: Jun 24, 2026

A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells

A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells

Published on: April 11, 2014

Improved UPLC-UV Method for the Quantification of Vitamin C in Lettuce Varieties (Lactuca sativa L.) and Crop Wild Relatives (Lactuca spp.)

Improved UPLC-UV Method for the Quantification of Vitamin C in Lettuce Varieties (Lactuca sativa L.) and Crop Wild Relatives (Lactuca spp.)

Published on: June 30, 2020

Examination of Rapid Dopamine Dynamics with Fast Scan Cyclic Voltammetry During Intra-oral Tastant Administration in Awake Rats

Examination of Rapid Dopamine Dynamics with Fast Scan Cyclic Voltammetry During Intra-oral Tastant Administration in Awake Rats

Published on: August 12, 2015

Area of Science:

Electrochemistry
Analytical Chemistry
Food Science

Background:

Ascorbic acid (vitamin C) is a vital nutrient and antioxidant.
Accurate quantification of ascorbic acid in fruit juices is crucial for quality control and consumer information.
Existing methods for ascorbic acid determination may have limitations in speed or accuracy.

Purpose of the Study:

To develop and validate a novel method for quantifying ascorbic acid in commercial fruit juices using cyclic voltammetry.
To establish the linearity, sensitivity, and precision of the developed electrochemical method.
To compare the results obtained by cyclic voltammetry with a standard volumetric method.

Main Methods:

Cyclic voltammetry was employed using a platinum disc electrode.
The anodic oxidation peak of ascorbic acid was analyzed at approximately 490 mV versus SCE.
Calibration curves were generated by varying ascorbic acid concentrations (0.1-10 mmol/L) and potential sweep speeds.

Main Results:

A linear relationship was observed between peak height and ascorbic acid concentration (R² = 0.9995).
The method demonstrated high precision (R.S.D. = 1.14%) and recovery rates between 94.35% and 104%.
Ascorbic acid levels in orange, lemon, and grapefruit juices were successfully determined, showing good agreement with volumetric analysis.

Conclusions:

Cyclic voltammetry offers a reliable and accurate method for determining ascorbic acid concentration in fruit juices.
The developed electrochemical technique is suitable for routine quality control and analysis in the food industry.
This method provides a valuable alternative to traditional analytical techniques for vitamin C assessment.