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Updated: Jun 24, 2026
06:24
Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology
Published on: December 15, 2017
Development of fundamental technologies for micro bioreactors
Kiichi Sato1, Takehiko Kitamori
1Department of Applied Biological Chemistry, The University of Tokyo, Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan, analchem@mail.ecc.u-tokyo.ac.jp.
Advances in Biochemical Engineering/Biotechnology
|April 4, 2009
Summary
This review covers essential technologies for microchip bioreactors using mammalian cells and pressure-driven flow. Key factors include cell culture, microfluidic medium supply, surface modification, and controlled flow rates to manage shear stress.
Area of Science:
- Biotechnology
- Cell Biology
- Microfluidics
Background:
- Microchip-based bioreactors offer advanced cell culture capabilities.
- Mammalian cell culture in microfluidic devices requires specific technological advancements.
Purpose of the Study:
- To review the fundamental technologies for developing microchip-based bioreactors.
- To highlight critical parameters for successful mammalian cell culture in microfluidic systems.
Main Methods:
- Discussion of microfluidic channel design for continuous medium supply.
- Exploration of surface modification techniques for cell attachment.
- Analysis of flow rate optimization to mitigate shear stress effects on cells.
Main Results:
- Continuous medium supply and appropriate surface modification are crucial for cell culture.
- Careful control of medium flow rate is necessary to manage shear stress.
- Developed technologies enable stable mammalian cell culture in microfluidic environments.
Conclusions:
- The reviewed technologies are fundamental for microchip bioreactor development.
- These advancements support applications in artificial organs, pigment production, and artificial insemination.
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