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High-definition Fourier Transform Infrared (FT-IR) Spectroscopic Imaging of Human Tissue Sections towards Improving Pathology
Published on: January 21, 2015
UVB radiation induced effects on cells studied by FTIR spectroscopy
Lucia Di Giambattista1, P Grimaldi, S Gaudenzi
1Dipartimento di Fisica, Università di Roma Sapienza, Piazzale A. Moro 2, Rome, Italy. l.digiambattista@caspur.it
European Biophysics Journal : EBJ
|April 4, 2009
Summary
UVB radiation impacts tumoral Jurkat cells. Fourier transform infrared spectroscopy (FTIR) and flow cytometry revealed significant spectral changes in proteins and nucleic acids, indicating cellular damage.
Area of Science:
- Biomedical Science
- Cell Biology
- Spectroscopy
Background:
- UVB radiation is known to induce DNA damage and apoptosis in cells.
- Fourier transform infrared spectroscopy (FTIR) offers a non-invasive method to analyze biomolecular composition.
- Flow cytometry is a standard technique for assessing cell viability and apoptosis.
Purpose of the Study:
- To investigate the effects of UVB radiation on the lymphoid T cell line Jurkat.
- To analyze cellular changes using FTIR spectroscopy and flow cytometry.
- To correlate spectroscopic markers with apoptosis induction.
Main Methods:
- Jurkat cells were exposed to UVB radiation (310 mJ/cm(2)) with and without a vegetable mixture.
- Fourier transform infrared spectroscopy (FTIR) was used to analyze molecular composition.
- Flow cytometry was employed to measure cytotoxicity and apoptosis percentages.
Main Results:
- Significant changes in absorbance and spectral patterns were observed in the protein and nucleic acid regions after UVB treatment.
- FTIR spectroscopy identified distinct spectral shifts indicative of cellular alterations.
- Flow cytometry confirmed UVB-induced cytotoxicity and apoptosis.
Conclusions:
- FTIR spectroscopy can detect UVB-induced molecular changes in Jurkat cells.
- Spectroscopic markers correlate with apoptosis, providing insights into cellular response to UVB.
- This study highlights the potential of FTIR as a complementary tool for apoptosis assessment.
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