Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 24, 2026

Novel Sequence Discovery by Subtractive Genomics
09:40

Novel Sequence Discovery by Subtractive Genomics

Published on: January 25, 2019

Solid phase subtractive cloning in differentially expressed genes identification.

Oscar F D'Urso1, Pietro I D'Urso, Carlo Storelli

  • 1Promeridio Association, Non-Profit Research Association, San Michele Salentino, BR, Italy. doscarferna@gmail.com

Molecular Biology Reports
|April 4, 2009
PubMed
Summary

We created a new high-throughput subtraction system for efficiently identifying specific DNA. This method immobilizes RNA on slides, simplifying the isolation of target DNA sequences for cloning.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Correction: Didactic adaptation of basketball in physical education to reduce anxiety and emotional eating in adolescent girls with obesity: a randomized controlled trial.

Frontiers in psychology·2026
Same author

Didactic adaptation of basketball in physical education to reduce anxiety and emotional eating in adolescent girls with obesity: a randomized controlled trial.

Frontiers in psychology·2026
Same author

Cardiorespiratory and Hematological Responses to High-Intensity Interval Training in Adolescent Girls With Overweight or Obesity: A Randomized Controlled Trial.

European journal of sport science·2026
Same author

Exercise-Induced Improvements in Renal and Inflammatory Markers in Postmenopausal Women With Obesity: A Randomized Study.

Women's health issues : official publication of the Jacobs Institute of Women's Health·2026
Same author

Effects of external verbal encouragement during minifootball on affective responses and post-training working memory in male adolescents.

Acta psychologica·2026
Same author

The neutrophil-to-lymphocyte ratio as a marker of immunosenescence and COVID-19 outcomes in the elderly: A narrative review.

Physiological reports·2026

Area of Science:

  • Molecular Biology
  • Genomics
  • Biotechnology

Background:

  • Gene expression analysis requires methods to identify unique sequences between sample sets.
  • Traditional subtraction techniques can be time-consuming and labor-intensive.
  • High-throughput methods are needed for efficient screening of large numbers of samples.

Purpose of the Study:

  • To develop a novel array-based subtractive hybridization system.
  • To enable one-step, high-throughput subtraction for gene discovery.
  • To facilitate the efficient isolation and cloning of target-specific cDNA.

Main Methods:

  • Development of an array-based system utilizing subtractive hybridization.
  • Immobilization of subtractor RNA onto a slide surface at high density (up to 10,000x).

More Related Videos

Functional Cloning Using a Xenopus Oocyte Expression System
09:40

Functional Cloning Using a Xenopus Oocyte Expression System

Published on: January 30, 2016

Related Experiment Videos

Last Updated: Jun 24, 2026

Novel Sequence Discovery by Subtractive Genomics
09:40

Novel Sequence Discovery by Subtractive Genomics

Published on: January 25, 2019

Functional Cloning Using a Xenopus Oocyte Expression System
09:40

Functional Cloning Using a Xenopus Oocyte Expression System

Published on: January 30, 2016

  • Hybridization of cDNA from target and subtractor samples, followed by retrieval and cloning of unbound target-specific cDNA.

    • Main Results:

    • Achieved an excellent contact surface area using a minimal amount of immobilized RNA.
    • Demonstrated a one-step process for high-throughput subtraction.
    • Successfully isolated target-specific cDNA, leaving common sequences bound to the immobilized RNA.

    Conclusions:

    • The developed array-based subtractive hybridization system offers an efficient and high-throughput solution for identifying unique DNA sequences.
    • This method simplifies the process of gene discovery by enabling direct cloning of target-specific cDNA.
    • The system's ability to achieve high density immobilization and one-step processing makes it valuable for various genomic applications.