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Electron Microscope Tomography and Single-particle Reconstruction01:07

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Related Experiment Video

Updated: Jun 24, 2026

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
12:51

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

Clinical multiphoton tomography.

Karsten König1

  • 1Faculty of Mechatronics and Physics, Saarland University, Campus, 66123 Saarbrücken, Germany. k.koenig@uni-saarland.de

Journal of Biophotonics
|April 4, 2009
PubMed
Summary

Clinical multiphoton tomography and two-photon microendoscopy offer high-resolution in vivo optical biopsies. These advanced molecular imaging tools are revolutionizing early disease detection and product screening.

Area of Science:

  • Biomedical Optics
  • In Vivo Imaging
  • Molecular Imaging

Background:

  • Multiphoton tomography and two-photon microendoscopy are advanced optical techniques.
  • These methods utilize two-photon autofluorescence, second harmonic generation, and fluorescence lifetime imaging.
  • They enable high-resolution in vivo optical biopsies.

Purpose of the Study:

  • To review the state-of-the-art technology in clinical multiphoton tomography and two-photon microendoscopy.
  • To report on the diverse applications of these molecular imaging tools.
  • To highlight their potential in various medical and commercial fields.

Main Methods:

  • Review of current technological advancements in multiphoton tomography and two-photon microendoscopy.
  • Analysis of applications including early-stage melanoma detection, skin aging studies, nanoparticle imaging, tissue engineering, and product screening.

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Last Updated: Jun 24, 2026

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Published on: September 30, 2021

  • Compilation of data from over 500 patient and volunteer investigations across Europe, Asia, and Australia.

    • Main Results:

    • Demonstration of high-resolution in vivo optical biopsies using advanced optical techniques.
    • Successful application in detecting early-stage melanoma and assessing skin aging.
    • Validation of utility in nanoparticle imaging, tissue engineering, and in situ screening of pharmaceuticals and cosmetics.
    • Successful investigation in over 500 patients and volunteers globally.

    Conclusions:

    • Clinical multiphoton tomography and two-photon microendoscopy represent cutting-edge molecular imaging tools.
    • These technologies offer significant potential for non-invasive diagnostics and product evaluation.
    • The broad range of successful applications underscores their clinical and commercial value.