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Related Concept Videos

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

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Related Experiment Video

Updated: Jun 24, 2026

Mapping Molecular Diffusion in the Plasma Membrane by Multiple-Target Tracing (MTT)
12:19

Mapping Molecular Diffusion in the Plasma Membrane by Multiple-Target Tracing (MTT)

Published on: May 27, 2012

Tracking bio-molecules in live cells using quantum dots.

Yun-Pei Chang1, Fabien Pinaud, Joshua Antelman

  • 1Department of Chemistry and Biochemistry, University of California at Los Angeles, 607 Charles Young Drive, East Los Angeles, California 90095-1569, USA. ypchang@chem.ucla.edu

Journal of Biophotonics
|April 4, 2009
PubMed
Summary
This summary is machine-generated.

Quantum dots (Qdots) are advanced probes for single particle tracking (SPT) in live cells. Recent surface coating and bio-conjugation improvements enhance their utility for studying biomolecule dynamics with high resolution.

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Last Updated: Jun 24, 2026

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Published on: May 27, 2012

Production and Targeting of Monovalent Quantum Dots
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Production and Targeting of Monovalent Quantum Dots

Published on: October 23, 2014

High-throughput Confocal Imaging of Quantum Dot-Conjugated SARS-CoV-2 Spike Trimers to Track Binding and Endocytosis in HEK293T Cells
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High-throughput Confocal Imaging of Quantum Dot-Conjugated SARS-CoV-2 Spike Trimers to Track Binding and Endocytosis in HEK293T Cells

Published on: April 21, 2022

Area of Science:

  • Biophysics
  • Cell Biology
  • Nanotechnology

Background:

  • Single particle tracking (SPT) is crucial for understanding biomolecule dynamics in live cells.
  • Achieving single-molecule sensitivity and nanometer spatial resolution is key for detailed cellular analysis.

Purpose of the Study:

  • To review recent advancements in quantum dot (Qdot) applications for SPT in live cell experiments.
  • To highlight the suitability of Qdots as probes for live cell studies.

Main Methods:

  • Review of recent literature on quantum dot surface coating techniques.
  • Analysis of novel bio-conjugation strategies for Qdots.
  • Evaluation of Qdots in the context of single particle tracking (SPT) experiments.

Main Results:

  • Quantum dots (Qdots) have undergone significant improvements in surface coating and bio-conjugation.
  • These advancements make Qdots highly suitable probes for live cell applications.
  • Qdots enable high-resolution, single-molecule sensitivity tracking of biomolecules.

Conclusions:

  • Quantum dots are increasingly effective tools for live cell single particle tracking (SPT).
  • Continued development in Qdot technology promises further insights into cellular dynamics.
  • This review underscores the importance of Qdots in advancing live cell imaging and analysis.