Related Experiment Video
Updated: Jan 3, 2026

Simultaneous Label-Free Autofluorescence Multi-Harmonic Microscopy
Published on: August 29, 2025
Three-dimensional (3D) backward and forward second harmonic generation (SHG) microscopy of biological tissues
Paolo Bianchini1, Alberto Diaspro
1LAMBS, MicroScoBiO Research Center and Department of Physics, University of Genoa, Via Dodecaneso 33, 16146 Genoa, Italy.
Abstract:
In this work we aim to show how it is possible to exploit the second harmonic generation (SHG) signal for producing multimodal microscopic images of biological tissues. SHG microscopy constitutes an important tool for high-resolution, high-contrast, three-dimensional studies of live cell and tissue architectures. The physical origins of SHG within these tissues are addressed and reported in a comprehensive image gallery. Although SHG is a coherent process, the multiple scattering of tissue samples determines the ability to acquire signal in both backward and forward direction. We discuss here some key elements related to the backward and forward SHG signal in terms of acquisition architecture and related microscopic imaging.
More Related Videos
Related Concept Videos
Two-Dimensional Microscopy in Microbiology
Three-Dimensional Microscopy in Microbiology
Super-resolution Fluorescence Microscopy
Electron Microscope Tomography and Single-particle Reconstruction
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
Confocal Fluorescence Microscopy

